Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jung, J.H.; Lee, S.B.
    Identification and characterization of Thermoplasma acidophilum glyceraldehyde dehydrogenase: a new class of NADP+-specific aldehyde dehydrogenase (2006), Biochem. J., 397, 131-138.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.89 2-mercaptoethanol 1 mM, activity is enhanced more than 2fold Thermoplasma acidophilum
1.2.1.89 4-chloromercuribenzoic acid 1 mM, completely inhibits activity Thermoplasma acidophilum
1.2.1.89 4-hydroxymercuribenzoic acid 1 mM, completely inhibits activity Thermoplasma acidophilum
1.2.1.89 DTT 1 mM, activity is enhanced more than 2fold Thermoplasma acidophilum
1.2.1.89 glutathione 1 mM, activity is enhanced more than 2fold Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.89 Ca2+ 1 mM, 12 h, 4°C, complete loss of activity Thermoplasma acidophilum
1.2.1.89 Cd2+ 1 mM, 12 h, 4°C, 93% loss of activity Thermoplasma acidophilum
1.2.1.89 Cu2+ 1 mM, 12 h, 4°C, complete loss of activity Thermoplasma acidophilum
1.2.1.89 Hg2+ 1 mM, 12 h, 4°C, 99% loss of activity Thermoplasma acidophilum
1.2.1.89 Mg2+ 1 mM, 12 h, 4°C, 24% loss of activity Thermoplasma acidophilum
1.2.1.89 Mn2+ 1 mM, 12 h, 4°C, 44% loss of activity Thermoplasma acidophilum
1.2.1.89 Ni2+ 1 mM, 12 h, 4°C, 58% loss of activity Thermoplasma acidophilum
1.2.1.89 Zn2+ 1 mM, 12 h, 4°C, 46% loss of activity Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.89 0.017
-
 NADP+ pH 8.0, 50°C Thermoplasma acidophilum
1.2.1.89 0.33
-
 D-glyceraldehyde pH 8.0, 50°C Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.89 53000
-
 4 * 53000, SDS-PAGE Thermoplasma acidophilum
1.2.1.89 54782
-
 4 * 54782, calculated from sequence Thermoplasma acidophilum
1.2.1.89 215000
-
 gel filtration Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.89 D-glyceraldehyde + NADP+ + H2O Thermoplasma acidophilum the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O Thermoplasma acidophilum JCM 9062 the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway D-glycerate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.89 Thermoplasma acidophilum Q9HK01
-
-
1.2.1.89 Thermoplasma acidophilum JCM 9062 Q9HK01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.89
-
 Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.89 2.85
-
 pH 8.0, 50°C Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.89 D-glyceraldehyde + NADP+ + H2O the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway Thermoplasma acidophilum D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O completely inactive when NAD+ is substituted for NADP+ Thermoplasma acidophilum D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway Thermoplasma acidophilum JCM 9062 D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O completely inactive when NAD+ is substituted for NADP+ Thermoplasma acidophilum JCM 9062 D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde 3-phosphate + NADP+ + H2O activity is 33% compared to the activity with D-glyceraldehyde Thermoplasma acidophilum D-glycerate 3-phosphate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde 3-phosphate + NADP+ + H2O activity is 33% compared to the activity with D-glyceraldehyde Thermoplasma acidophilum JCM 9062 D-glycerate 3-phosphate + NADPH + H+
-
 ?
1.2.1.89 glycolaldehyde + NADP+ activity is 14% compared to the activity with D-glyceraldehyde Thermoplasma acidophilum glycolate + NADPH + H+
-
 ?
1.2.1.89 glycolaldehyde + NADP+ activity is 14% compared to the activity with D-glyceraldehyde Thermoplasma acidophilum JCM 9062 glycolate + NADPH + H+
-
 ?
1.2.1.89 additional information no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde) Thermoplasma acidophilum ?
-
 ?
1.2.1.89 additional information no activity towards ketoaldehyde (methylglyoxal), alkyl aldehydes (formaldehyde, acetaldehyde and propionaldehyde) or aromatic aldehydes (phenylacetaldehyde and 4-nitrobenzaldehyde) Thermoplasma acidophilum JCM 9062 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.89 homotetramer 4 * 53000, SDS-PAGE Thermoplasma acidophilum
1.2.1.89 tetramer 4 * 54782, calculated from sequence Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
1.2.1.89 GLD
-
 Thermoplasma acidophilum
1.2.1.89 glyceraldehyde dehydrogenase
-
 Thermoplasma acidophilum
1.2.1.89 Ta0809 locus name Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.89 50 55
-
 Thermoplasma acidophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.89 30 70 30°C: about 50% of maximal activity, 70°C: about 60% of maximal activity Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.89 60
-
 stable for 2 h Thermoplasma acidophilum
1.2.1.89 80
-
 activity is decreased to less than 50% within 20 min Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.89 8
-
-
 Thermoplasma acidophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.89 6 10 pH 6.0: about 65% of maximal activity, pH 10.0: about 70% of maximal activity Thermoplasma acidophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.89 6 8 24 h, stable Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.89 NADP+ completely inactive when NAD+ is substituted for NADP+ Thermoplasma acidophilum

General Information

EC Number General Information Comment Organism
1.2.1.89 physiological function the enzyme is involved in downstream portion of the non-phosphorylated Entner–Doudoroff pathway Thermoplasma acidophilum