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Literature summary extracted from

  • Peterbauer, C.; Volc, J.
    Pyranose dehydrogenases: Biochemical features and perspectives of technological applications (2010), Appl. Microbiol. Biotechnol., 85, 837-848.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.54
-
L-arabinose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 0.82
-
D-glucose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 1.05
-
D-galactose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 1.93
-
D-xylose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 6.82
-
cellobiose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 8.61
-
D-maltose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 10.8
-
xylobiose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 59.7
-
D-ribose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 79.1
-
D-talose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 108
-
D-mannose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 134
-
lactose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 137
-
L-sorbose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 153
-
D-fructose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 156
-
maltotriose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.29 extracellular
-
Chlorophyllum rhacodes
-
-
1.1.99.29 extracellular
-
Agaricus xanthodermus
-
-
1.1.99.29 extracellular
-
Leucoagaricus meleagris
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 75000
-
1 * 75000 Chlorophyllum rhacodes
1.1.99.29 75000
-
1 * 75000 Agaricus xanthodermus
1.1.99.29 75000
-
1 * 75000 Leucoagaricus meleagris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.29 2,3-didehydro-D-glucose + 1,4-benzoquinone Chlorophyllum rhacodes
-
3-dehydro-D-glucose + hydroquinone
-
?
1.1.99.29 2,3-didehydro-D-glucose + 1,4-benzoquinone Agaricus xanthodermus
-
3-dehydro-D-glucose + hydroquinone
-
?
1.1.99.29 2,3-didehydro-D-glucose + 1,4-benzoquinone Leucoagaricus meleagris
-
3-dehydro-D-glucose + hydroquinone
-
?
1.1.99.29 2-dehydro-D-xylose + 1,4-benzoquinone Chlorophyllum rhacodes
-
2,3-didehydro-D-xylose + hydroquinone
-
?
1.1.99.29 2-dehydro-D-xylose + 1,4-benzoquinone Agaricus xanthodermus
-
2,3-didehydro-D-xylose + hydroquinone
-
?
1.1.99.29 2-dehydro-D-xylose + 1,4-benzoquinone Leucoagaricus meleagris
-
2,3-didehydro-D-xylose + hydroquinone
-
?
1.1.99.29 a pyranoside + acceptor Chlorophyllum rhacodes
-
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
?
1.1.99.29 a pyranoside + acceptor Agaricus xanthodermus
-
a 3-dehydropyranoside + reduced acceptor
-
?
1.1.99.29 a pyranoside + acceptor Leucoagaricus meleagris
-
a 3-dehydropyranoside + reduced acceptor
-
?
1.1.99.29 cellobiose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 D-fructose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 D-galactose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 D-glucose + 1,4-benzoquinone Chlorophyllum rhacodes
-
2,3-didehydro-D-glucose + hydroquinone
-
?
1.1.99.29 D-glucose + 1,4-benzoquinone Agaricus xanthodermus
-
2,3-didehydro-D-glucose + hydroquinone
-
?
1.1.99.29 D-glucose + 1,4-benzoquinone Leucoagaricus meleagris
-
2,3-didehydro-D-glucose + hydroquinone
-
?
1.1.99.29 D-glucose + ferricenium ion Chlorophyllum rhacodes
-
3-dehydro-D-glucose + ferrocene
-
?
1.1.99.29 D-glucose + ferricenium ion Agaricus xanthodermus
-
3-dehydro-D-glucose + ferrocene
-
?
1.1.99.29 D-glucose + ferricenium ion Leucoagaricus meleagris
-
3-dehydro-D-glucose + ferrocene
-
?
1.1.99.29 D-maltose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 D-mannose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 D-ribose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 D-talose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 D-xylose + 1,4-benzoquinone Chlorophyllum rhacodes
-
2-dehydro-D-xylose + hydroquinone
-
?
1.1.99.29 D-xylose + 1,4-benzoquinone Agaricus xanthodermus
-
2-dehydro-D-xylose + hydroquinone
-
?
1.1.99.29 D-xylose + 1,4-benzoquinone Leucoagaricus meleagris
-
2-dehydro-D-xylose + hydroquinone
-
?
1.1.99.29 D-xylose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 L-arabinose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 L-sorbose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 lactose + acceptor Chlorophyllum rhacodes
-
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
-
?
1.1.99.29 lactose + acceptor Agaricus xanthodermus
-
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
-
?
1.1.99.29 lactose + acceptor Leucoagaricus meleagris
-
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
-
?
1.1.99.29 lactose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 maltotriose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?
1.1.99.29 pyranose + acceptor Chlorophyllum rhacodes
-
2,3-didehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor Agaricus xanthodermus
-
2,3-didehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor Leucoagaricus meleagris
-
2,3-didehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor Chlorophyllum rhacodes
-
2-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor Agaricus xanthodermus
-
2-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor Leucoagaricus meleagris
-
2-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor Chlorophyllum rhacodes
-
3-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor Leucoagaricus meleagris
-
3-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranoside + acceptor Agaricus xanthodermus
-
3,4-didehydropyranoside + reduced acceptor
-
?
1.1.99.29 pyranoside + acceptor Leucoagaricus meleagris
-
3,4-didehydropyranoside + reduced acceptor
-
?
1.1.99.29 xylobiose + ferricenium ion Leucoagaricus meleagris
-
? + ferrocene
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Agaricus xanthodermus Q3L1D2
-
-
1.1.99.29 Chlorophyllum rhacodes
-
-
-
1.1.99.29 Leucoagaricus meleagris Q3L245
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein
-
Chlorophyllum rhacodes
1.1.99.29 glycoprotein
-
Agaricus xanthodermus
1.1.99.29 glycoprotein
-
Leucoagaricus meleagris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 2,3-didehydro-D-glucose + 1,4-benzoquinone
-
Chlorophyllum rhacodes 3-dehydro-D-glucose + hydroquinone
-
?
1.1.99.29 2,3-didehydro-D-glucose + 1,4-benzoquinone
-
Agaricus xanthodermus 3-dehydro-D-glucose + hydroquinone
-
?
1.1.99.29 2,3-didehydro-D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris 3-dehydro-D-glucose + hydroquinone
-
?
1.1.99.29 2-dehydro-D-xylose + 1,4-benzoquinone
-
Chlorophyllum rhacodes 2,3-didehydro-D-xylose + hydroquinone
-
?
1.1.99.29 2-dehydro-D-xylose + 1,4-benzoquinone
-
Agaricus xanthodermus 2,3-didehydro-D-xylose + hydroquinone
-
?
1.1.99.29 2-dehydro-D-xylose + 1,4-benzoquinone
-
Leucoagaricus meleagris 2,3-didehydro-D-xylose + hydroquinone
-
?
1.1.99.29 a pyranoside + acceptor
-
Chlorophyllum rhacodes a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
?
1.1.99.29 a pyranoside + acceptor
-
Agaricus xanthodermus a 3-dehydropyranoside + reduced acceptor
-
?
1.1.99.29 a pyranoside + acceptor
-
Leucoagaricus meleagris a 3-dehydropyranoside + reduced acceptor
-
?
1.1.99.29 cellobiose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-fructose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-galactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
Chlorophyllum rhacodes 2,3-didehydro-D-glucose + hydroquinone
-
?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
Agaricus xanthodermus 2,3-didehydro-D-glucose + hydroquinone
-
?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
Leucoagaricus meleagris 2,3-didehydro-D-glucose + hydroquinone
-
?
1.1.99.29 D-glucose + ferricenium ion
-
Chlorophyllum rhacodes 3-dehydro-D-glucose + ferrocene
-
?
1.1.99.29 D-glucose + ferricenium ion
-
Agaricus xanthodermus 3-dehydro-D-glucose + ferrocene
-
?
1.1.99.29 D-glucose + ferricenium ion
-
Leucoagaricus meleagris 3-dehydro-D-glucose + ferrocene
-
?
1.1.99.29 D-maltose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-mannose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-ribose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-talose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 D-xylose + 1,4-benzoquinone
-
Chlorophyllum rhacodes 2-dehydro-D-xylose + hydroquinone
-
?
1.1.99.29 D-xylose + 1,4-benzoquinone
-
Agaricus xanthodermus 2-dehydro-D-xylose + hydroquinone
-
?
1.1.99.29 D-xylose + 1,4-benzoquinone
-
Leucoagaricus meleagris 2-dehydro-D-xylose + hydroquinone
-
?
1.1.99.29 D-xylose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 L-arabinose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 L-sorbose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 lactose + acceptor
-
Chlorophyllum rhacodes lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
-
?
1.1.99.29 lactose + acceptor
-
Agaricus xanthodermus lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
-
?
1.1.99.29 lactose + acceptor
-
Leucoagaricus meleagris lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
-
?
1.1.99.29 lactose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 maltotriose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?
1.1.99.29 pyranose + acceptor
-
Chlorophyllum rhacodes 2,3-didehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor
-
Agaricus xanthodermus 2,3-didehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor
-
Leucoagaricus meleagris 2,3-didehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor
-
Chlorophyllum rhacodes 2-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor
-
Agaricus xanthodermus 2-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor
-
Leucoagaricus meleagris 2-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor
-
Chlorophyllum rhacodes 3-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranose + acceptor
-
Leucoagaricus meleagris 3-dehydropyranose + reduced acceptor
-
?
1.1.99.29 pyranoside + acceptor
-
Agaricus xanthodermus 3,4-didehydropyranoside + reduced acceptor
-
?
1.1.99.29 pyranoside + acceptor
-
Leucoagaricus meleagris 3,4-didehydropyranoside + reduced acceptor
-
?
1.1.99.29 xylobiose + ferricenium ion
-
Leucoagaricus meleagris ? + ferrocene
-
?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 monomer 1 * 75000 Chlorophyllum rhacodes
1.1.99.29 monomer 1 * 75000 Agaricus xanthodermus
1.1.99.29 monomer 1 * 75000 Leucoagaricus meleagris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH
-
Chlorophyllum rhacodes
1.1.99.29 PDH
-
Agaricus xanthodermus
1.1.99.29 PDH
-
Leucoagaricus meleagris
1.1.99.29 pyranose:acceptor oxidoreductase
-
Chlorophyllum rhacodes
1.1.99.29 pyranose:acceptor oxidoreductase
-
Agaricus xanthodermus
1.1.99.29 pyranose:acceptor oxidoreductase
-
Leucoagaricus meleagris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.29 15.7
-
L-sorbose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 16.5
-
D-fructose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 21.3
-
D-talose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 23.6
-
maltotriose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 29.3
-
D-mannose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 31.4
-
D-ribose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 37.2
-
L-arabinose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 38.4
-
cellobiose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 39.6
-
lactose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 43.2
-
D-maltose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 43.4
-
D-xylose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 45.9
-
D-glucose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 48.5
-
D-galactose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 50.4
-
xylobiose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.29 0.11
-
D-fructose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 0.11
-
L-sorbose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 0.15
-
maltotriose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 0.27
-
D-mannose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 0.27
-
D-talose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 0.29
-
lactose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 0.52
-
D-ribose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 4.7
-
xylobiose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 5
-
D-maltose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 5.6
-
cellobiose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 22.9
-
D-xylose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 46.2
-
D-galactose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 57.5
-
D-glucose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris
1.1.99.29 62.1
-
L-arabinose with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication Leucoagaricus meleagris