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Literature summary extracted from
- Purification and characterization of an anabolic fumarate reductase from Methanobacterium thermoautotrophicum (1989), Appl. Environ. Microbiol., 55, 856-861.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.4.1 | CuCl2 | 5 mM, about 80% loss of activity | Methanothermobacter thermautotrophicus |  |
| 1.3.4.1 | iodoacetamide | 1 mM, about 80% loss of activity after 20 min | Methanothermobacter thermautotrophicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.4.1 | 0.2 | - |
fumarate | pH 7.0, 75°C | Methanothermobacter thermautotrophicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.4.1 | cytosol | - |
Methanothermobacter thermautotrophicus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.4.1 | 20000 | - |
x * 20000, SDS-PAGE | Methanothermobacter thermautotrophicus |
| 1.3.4.1 | 78000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.4.1 | fumarate + CoM + CoB | Methanothermobacter thermautotrophicus | - |
succinate + CoM-S-S-CoB | - |
? | |
| 1.3.4.1 | fumarate + CoM + CoB | Methanothermobacter thermautotrophicus DELTAH | - |
succinate + CoM-S-S-CoB | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.4.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
| 1.3.4.1 | Methanothermobacter thermautotrophicus DELTAH | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.3.4.1 | very oxygen sensitive | Methanothermobacter thermautotrophicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.4.1 | - |
Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.4.1 | fumarate + CoM + CoB | - |
Methanothermobacter thermautotrophicus | succinate + CoM-S-S-CoB | - |
? | |
| 1.3.4.1 | fumarate + CoM + CoB | the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor | Methanothermobacter thermautotrophicus | succinate + CoM-S-S-CoB | - |
? | |
| 1.3.4.1 | fumarate + CoM + CoB | - |
Methanothermobacter thermautotrophicus DELTAH | succinate + CoM-S-S-CoB | - |
? | |
| 1.3.4.1 | fumarate + CoM + CoB | the enzyme cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor | Methanothermobacter thermautotrophicus DELTAH | succinate + CoM-S-S-CoB | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.4.1 | ? | x * 20000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.4.1 | Tfr | - |
Methanothermobacter thermautotrophicus |
| 1.3.4.1 | thiol:fumarate reductase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.4.1 | 75 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.4.1 | 40 | 80 | 40°C: about 40% of maximal activity, 80°C: about 50% of maximal activity | Methanothermobacter thermautotrophicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.4.1 | 70 | - |
no detectable loss in the activity is observed when the enzyme (0.03 mg of protein per ml) is incubated anaerobically at 70°C in sodium phosphate buffer (pH 7.0) containing 1 mM dithiothreitol and 15 mM 2-mercaptoethanol for 8 h | Methanothermobacter thermautotrophicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.4.1 | 7 | - |
- |
Methanothermobacter thermautotrophicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.4.1 | additional information | absence of flavins as a cofactor. The spectral data, suggests the presence of an unknown cofactor tightly bound to the enzyme | Methanothermobacter thermautotrophicus |
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