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Literature summary extracted from

  • Bachas-Daunert, P.G.; Sellers, Z.P.; Wei, Y.
    Detection of halogenated organic compounds using immobilized thermophilic dehalogenase (2009), Anal. Bioanal. Chem., 395, 1173-1178.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.8.1.2 analysis the enzyme is covalently linked to an N-hydroxysuccinimidyl Sepharose resin to construct a highly specific sensor with long shelf life for the detection of L-2-haloacids Sulfurisphaera tokodaii

General Stability

EC Number General Stability Organism
3.8.1.2 the enzyme retains approximately 30% of the overall activity after immobilization Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 1.2
-
 (S)-2-chloropropionic acid pH 9.0, 50°C, soluble enzyme Sulfurisphaera tokodaii
3.8.1.2 1.6
-
 (S)-2-chloropropionic acid pH 9.0, 22°C, immobilized enzyme Sulfurisphaera tokodaii
3.8.1.2 2.7
-
 (S)-2-chloropropionic acid pH 9.0, 37°C, immobilized enzyme Sulfurisphaera tokodaii
3.8.1.2 4.9
-
 (S)-2-chloropropionic acid pH 9.0, 50°C, immobilized enzyme Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.2 0.9
-
 substrate: (S)-2-chloropropionic acid, pH 9.0, 22°C, immobilized enzyme Sulfurisphaera tokodaii
3.8.1.2 3
-
 substrate: (S)-2-chloropropionic acid, pH 9.0, 37°C, immobilized enzyme Sulfurisphaera tokodaii
3.8.1.2 6.3
-
 substrate: (S)-2-chloropropionic acid, pH 9.0, 50°C, immobilized enzyme Sulfurisphaera tokodaii
3.8.1.2 20
-
 substrate: (S)-2-chloropropionic acid, pH 9.0, 50°C, soluble enzyme Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-chloropropionic acid + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.2 L-HADST
-
 Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 9.5
-
 soluble and immobilized enzyme Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.2 8 10.5 pH 8.0: about 50% of maximal activity, pH 10.5: about 80% of maximal activity Sulfurisphaera tokodaii