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Literature summary extracted from

  • Häkkinen, M.; Hyönen, M.; Auriola, S.; Casero Jr., R.; Vepsäläinen, J.; Khomutov, A.; Alhonen, L.; Keinänen, T.
    Metabolism of N-alkylated spermine analogues by polyamine and spermine oxidases (2010), Amino Acids, 38, 369-381.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.16 N1,N4-bis(2,3-butadienyl)-1,4 butanediamine i.e. MDL72527 Homo sapiens
1.5.3.16 spermidine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.13 additional information
-
 additional information kinetics of APAO with diverse polyamine analogues and their predicted secondary metabolites, overview Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.16 68000
-
 x * 68000 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.13 N,N'-bis-(3-benzylaminopropyl)butane-1,4-diamine + O2 + H2O Homo sapiens
-
 N1-(3-benzylaminopropyl)butane-1,4-diamine + N-(3-aminopropyl)-N'-(3-Benzylaminopropyl)butane-1,4-diamine + H2O2
-
 ?
1.5.3.13 N1,N12-diacetylspermine + O2 + H2O Homo sapiens
-
 N1-acetylspermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.13 N1-acetylspermidine + O2 + H2O Homo sapiens
-
 putrescine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.13 N1-acetylspermine + O2 + H2O Homo sapiens
-
 spermidine + 3-acetamidopropanal + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.13 Homo sapiens
-
-
-
1.5.3.16 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.13 DU-145 cell
-
 Homo sapiens
-
1.5.3.13 additional information APAO is constitutively expressed in various tissues Homo sapiens
-
1.5.3.16 DU-145 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.13 additional information APAO is capable of metabolizing several N-alkylated polyamine derivatives, substrate specificity, overview. N1-(3-Ethylaminopropyl)butane-1,4-diamine trihydrochloride is a poor substrate Homo sapiens ?
-
 ?
1.5.3.13 N,N'-bis-(3-benzylaminopropyl)butane-1,4-diamine + O2 + H2O
-
 Homo sapiens N1-(3-benzylaminopropyl)butane-1,4-diamine + N-(3-aminopropyl)-N'-(3-Benzylaminopropyl)butane-1,4-diamine + H2O2
-
 ?
1.5.3.13 N,N'-bis-(3-benzylaminopropyl)butane-1,4-diamine + O2 + H2O
-
 Homo sapiens N1-(3-benzylaminopropyl)butane-1,4-diamine + H2O2 + ? main product ?
1.5.3.13 N,N'-bis-(3-ethylaminopropyl)butane-1,4-diamine + O2 + H2O minor N4-endo cleavage pathways resulting in formation of N1-ethylpropane-1,3-diamine Homo sapiens N1-(3-ethylaminopropyl)butane-1,4-diamine + H2O2 + ?
-
 ?
1.5.3.13 N-(3-benzylaminopropyl)-N'-(3-ethylaminopropyl)butane-1,4-diamine + O2 + H2O
-
 Homo sapiens N1-(3-ethylaminopropyl)butane-1,4-diamine + H2O2 + ?
-
 ?
1.5.3.13 N1,N12-diacetylspermine + O2 + H2O
-
 Homo sapiens N1-acetylspermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.13 N1-(3-benzylaminopropyl)butane-1,4-diamine + O2 + H2O
-
 Homo sapiens spermidine + H2O2 + benzylalcohol
-
 ?
1.5.3.13 N1-acetylspermidine + O2 + H2O
-
 Homo sapiens putrescine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.13 N1-acetylspermine + O2 + H2O
-
 Homo sapiens spermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.13 N1-benzyl-propane-1,3-diamine + O2 + H2O
-
 Homo sapiens propane-1,3-diamine + H2O2 + benzylalcohol
-
 ?
1.5.3.16 additional information SMO is capable of metabolizing several N-alkylated polyamine derivatives, substrate specificity, overview. N1-(3-Ethylaminopropyl)butane-1,4-diamine trihydrochloride is a poor substrate Homo sapiens ?
-
 ?
1.5.3.16 N,N'-bis-(3-benzylaminopropyl)butane-1,4-diamine + O2 + H2O
-
 Homo sapiens N-(3-aminopropyl)-N'-(3-benzylaminopropyl)butane-1,4-diamine + N1-(3-benzylaminopropyl)butane-1,4-diamine + H2O2 + ? main products ?
1.5.3.16 N,N'-bis-(3-ethylaminopropyl)butane-1,4-diamine + O2 + H2O minor N4-endo cleavage pathways resulting in formation of EtDAP Homo sapiens N1-(3-ethylaminopropyl)butane-1,4-diamine + H2O2 + ?
-
 ?
1.5.3.16 N-(3-aminopropyl)-N-(3-ethylaminopropyl)butane-1,4-diamine + O2 + H2O
-
 Homo sapiens spermine + H2O2 + ? i.e. N,N'-bis-(3-aminopropyl)butane-1,4-diamine ?
1.5.3.16 N-(3-benzylaminopropyl)-N'-(3-ethylaminopropyl)butane-1,4-diamine + O2 + H2O
-
 Homo sapiens N1-(3-ethylaminopropyl)butane-1,4-diamine + H2O2 + ?
-
 ?
1.5.3.16 N1-acetylspermine + O2 + H2O the ethyl substituent that retains the amine as positively charged, strongly accelerates the reaction velocity Homo sapiens spermidine + 3-acetamidopropanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.16 ? x * 68000 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.5.3.13 APAO
-
 Homo sapiens
1.5.3.16 SMO
-
 Homo sapiens

Temperature Optimum [Ā°C]

EC Number Temperature Optimum [Ā°C] Temperature Optimum Maximum [Ā°C] Comment Organism
1.5.3.13 37
-
 assay at Homo sapiens
1.5.3.16 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.13 8
-
 assay at Homo sapiens
1.5.3.16 8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.13 FAD
-
 Homo sapiens
1.5.3.16 FAD
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
1.5.3.16 Homo sapiens no induction of SMO activity by N-alkylated polyamine analogues additional information

General Information

EC Number General Information Comment Organism
1.5.3.16 metabolism SMO is a key enzyme of polyamine catabolism, metabolism of N-alkylated spermine analogues by polyamine and spermine oxidases, overview Homo sapiens