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Literature summary extracted from

  • Georgescauld, F.; Moynie, L.; Habersetzer, J.; Cervoni, L.; Mocan, I.; Borza, T.; Harris, P.; Dautant, A.; Lascu, I.
    Intersubunit ionic interactions stabilize the nucleoside diphosphate kinase of Mycobacterium tuberculosis (2013), PLoS ONE, 8, e57867.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 mutant enzyme D93N is expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.6 mutant enzyme D93N crystallizes to hexagonal plates with 2.0 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, and to rods with 2.0 M ammonium sulfate, 2% (w/v) PEG400, 0.1 M HEPES, pH 7.5 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 D93N the mutation dramatically decreases protein thermal stability (Tm value of 43.4°C) but is still hexameric and retains full activity. The mutant hexamer is stable and active up to 2.5 M guanidine hydrochloride Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
2.7.4.6 guanidinium and many other salts stabilize the hexamer of mutant D93N with a half maximum effect of about 0.1 M, increasing protein thermostability Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.6 Urea inactivation occurs at very low urea concentrations (0.5 M) Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Mycobacterium tuberculosis P9WJH7
-
-
2.7.4.6 Mycobacterium tuberculosis H37Rv P9WJH7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 Q-Sepharose column chromatography Mycobacterium tuberculosis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.4.6 the wild type enzyme unfolds and inactivates simultaneously in the presence of 4 M guanidine hydrochloride as with 4 M urea as denaturant. Refolding occurs at much lower guanidine hydrochloride concentrations (0.5-1 M) Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + 8-bromoinosine 5'-diphosphate
-
 Mycobacterium tuberculosis ADP + 8-bromoinosine 5'-triphosphate
-
 ?
2.7.4.6 ATP + 8-bromoinosine 5'-diphosphate
-
 Mycobacterium tuberculosis H37Rv ADP + 8-bromoinosine 5'-triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDPK
-
 Mycobacterium tuberculosis
2.7.4.6 nucleoside diphosphate kinase
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.6 76
-
 the wild type enzyme has a temperature of half denaturation of 76°C Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.6 0.159
-
 8-bromoinosine 5'-diphosphate wild type enzyme, at pH 7.5 and 22°C Mycobacterium tuberculosis
2.7.4.6 0.23
-
 8-bromoinosine 5'-diphosphate mutant enzyme D93N, at pH 7.5 and 22°C Mycobacterium tuberculosis
2.7.4.6 232
-
 ATP mutant enzyme D93N, at pH 7.5 and 22°C Mycobacterium tuberculosis
2.7.4.6 264
-
 ATP wild type enzyme, at pH 7.5 and 22°C Mycobacterium tuberculosis