EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | phosphate | the enzyme is dependent on phosphate for its activity. The optimal concentration ranges from 10 mM to 40 mM | Mycobacterium tuberculosis | |
2.7.6.1 | SO42- | sulfate ions are able to stimulate the enzyme activity, but with respect to phosphate, are less effective. The optimal concentration ranges from 40 mM to 60 mM | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.6.1 | expressed in Escherichia coli BL21(DE3)pLysS cells | Mycobacterium tuberculosis |
EC Number | General Stability | Organism |
---|---|---|
2.7.6.1 | dialysis against buffers such as 50 mM Tris-HCl, pH 8.0 or 50 mM HEPES-NaOH, pH 8.0 without phosphate results in a protein precipitation and complete loss of activity. The addition of 50 mM ammonium sulfate or 5 mM Mg-ATP to Tris-HCl, pH 8.0 allows the enzyme to preserve 20% of initial activity after a period of 16 h, whereas full activity is maintained with the addition of 50 mM phosphate | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | ADP | strong inhibition | Mycobacterium tuberculosis | |
2.7.6.1 | Ca2+ | - |
Mycobacterium tuberculosis | |
2.7.6.1 | Cu2+ | - |
Mycobacterium tuberculosis | |
2.7.6.1 | Fe2+ | - |
Mycobacterium tuberculosis | |
2.7.6.1 | GDP | the enzyme is weakly sensitive to GDP | Mycobacterium tuberculosis | |
2.7.6.1 | additional information | no inhibitory effects are shown by the presence of pyrimidine nucleoside mono- or diphosphates or of histidine, up to 2 mM | Mycobacterium tuberculosis | |
2.7.6.1 | phosphate | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.06 | - |
D-ribose 5-phosphate | in the presence of 5 mM Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 0.07 | - |
D-ribose 5-phosphate | in the presence of 5 mM Mg2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 0.071 | - |
D-ribose 5-phosphate | in the absence of Mg2+ or Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | Mg2+ | allosterically activated by Mg2+ | Mycobacterium tuberculosis | |
2.7.6.1 | Mn2+ | allosterically activated by Mn2+ | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 35000 | - |
6 * 35000, SDS-PAGE | Mycobacterium tuberculosis |
2.7.6.1 | 220000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.6.1 | Mycobacterium tuberculosis | - |
- |
- |
2.7.6.1 | Mycobacterium tuberculosis Rv1017 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.6.1 | His-Trap HP column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | Mg-ATP2- + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | Mg-AMP2- + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | Mg-ATP2- + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis Rv1017 | Mg-AMP2- + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | Mn-ATP2- + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | Mn-AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | Mn-ATP2- + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis Rv1017 | Mn-AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.6.1 | homohexamer | 6 * 35000, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.6.1 | phosphoribosylpyrophosphate synthetase | - |
Mycobacterium tuberculosis |
2.7.6.1 | PRPPase | - |
Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 62 | 74 | a highly stable enzyme, losing 50% of its activity in 10 min of incubation at 62°C, and showing a Tm of 69.3°C (without ligands) to 74.5°C (in the presence of 5 mM Mg-ATP) | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 34.6 | - |
Mg-ATP2- | in the presence of 5 mM Mg2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 35.1 | - |
D-ribose 5-phosphate | in the presence of 5 mM Mg2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 35.5 | - |
Mg-ATP2- | in the absence of free Mg2+ or Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 37 | - |
D-ribose 5-phosphate | in the absence of Mg2+ or Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 44.3 | - |
Mg-ATP2- | in the presence of 5 mM Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 44.7 | - |
D-ribose 5-phosphate | in the presence of 5 mM Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 45.1 | - |
Mn-ATP2- | in the presence of 5 mM Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 46.3 | - |
Mn-ATP2- | in the absence of free Mg2+ or Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 8 | - |
the enzyme shows an optimum at a pH value close to 8.0 | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 7 | 9.5 | the enzyme possesses nearly 70% of its maximal activity at pH 9.5. The activity at pH 7 is 57% of the maximal one at pH 8.0 | Mycobacterium tuberculosis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.4 | - |
in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | GDP | |
2.7.6.1 | 5 | - |
in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | GDP | |
2.7.6.1 | 100 | - |
in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | phosphate |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.6.1 | physiological function | the enzyme is crucial for mycobacterial cell wall biosynthesis | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 501.4 | - |
D-ribose 5-phosphate | in the presence of 5 mM Mg2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 521 | - |
D-ribose 5-phosphate | in the absence of Mg2+ or Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis | |
2.7.6.1 | 745 | - |
D-ribose 5-phosphate | in the presence of 5 mM Mn2+, in 50 mM potassium phosphate pH 8.0, at 37°C | Mycobacterium tuberculosis |