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Literature summary extracted from

  • Lee, E.J.; Facchini, P.J.
    Tyrosine aminotransferase contributes to benzylisoquinoline alkaloid biosynthesis in opium poppy (2011), Plant Physiol., 157, 1067-1078.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.35
-
 2-oxoglutarate pH 8.5, 30°C Papaver somniferum
2.6.1.5 1.82
-
 L-tyrosine pH 8.5, 30°C Papaver somniferum
2.6.1.5 2.45
-
 pyruvate pH 8.5, 30°C Papaver somniferum
2.6.1.5 6.33
-
 L-phenylalanine pH 8.5, 30°C Papaver somniferum
2.6.1.5 7.83
-
 L-tryptophan pH 8.5, 30°C Papaver somniferum
2.6.1.5 56.13
-
 oxaloacetate pH 8.5, 30°C Papaver somniferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.5 46000
-
-
 Papaver somniferum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Papaver somniferum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.5 root transcripts highly detected Papaver somniferum
-
2.6.1.5 stem transcripts highly detected Papaver somniferum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-phenylalanine + 2-oxoglutarate
-
 Papaver somniferum 2-oxo-3-phenylpropanoate + L-glutamate
-
 ?
2.6.1.5 L-tryptophan + 2-oxoglutarate
-
 Papaver somniferum 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate
-
 ?
2.6.1.5 L-tryptophan + oxaloacetate
-
 Papaver somniferum 3-(1H-indol-3-yl)-2-oxopropanoate + L-glycine
-
 ?
2.6.1.5 L-tryptophan + pyruvate
-
 Papaver somniferum 3-(1H-indol-3-yl)-2-oxopropanoate + L-alanine
-
 ?
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Papaver somniferum 4-hydroxyphenylpyruvate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 TyrAT
-
 Papaver somniferum
2.6.1.5 tyrosine aminotransferase
-
 Papaver somniferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.5 30
-
 assay at Papaver somniferum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.5 0.08
-
 L-phenylalanine pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.22
-
 2-oxoglutarate pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.24
-
 L-tyrosine pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.34
-
 pyruvate pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.36
-
 L-tryptophan pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.37
-
 oxaloacetate pH 8.5, 30°C Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 8.5
-
-
 Papaver somniferum

General Information

EC Number General Information Comment Organism
2.6.1.5 malfunction virus-induced gene silencing is used to evaluate the contribution of TyrAT to benzylisoquinoline alkaloids metabolism in opium poppy. TyrAT transcript levels were reduced by at least 80% in silenced plants compared with controls and show a moderate reduction in total alkaloid content Papaver somniferum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.5 0.01
-
 oxaloacetate pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.01
-
 L-phenylalanine pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.13
-
 L-tyrosine pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.14
-
 pyruvate pH 8.5, 30°C Papaver somniferum
2.6.1.5 0.63
-
 2-oxoglutarate pH 8.5, 30°C Papaver somniferum