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Literature summary extracted from
- Molecular cloning and characterization of glucose-6-phosphate dehydrogenase from Brugia malayi (2013), Parasitology, 140, 897-906.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | guanosine hydrochloride | the enzyme shows a sharp loss in activity above 0.75 M, but is activated by low concentrations of 0.2 M of GdmCl | Brugia malayi |  |
| 1.1.1.49 | N-ethylmaleimide | blocking the SH group leads to activation of rBmG6PD activity | Brugia malayi | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.49 | recombinant expression of His-tagged enzyme in Escherichia coli strain C41 | Brugia malayi |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.49 | unfolding equilibrium of rBmG6PD with GdmCl/urea shows the triphasic unfolding pattern along with the highly stable intermediate obtained by GdmCl | Brugia malayi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | adenosine 5' [beta,gamma-amido] triphosphate | - |
Brugia malayi | |
| 1.1.1.49 | adenosine 5' [beta-thio] diphosphate | - |
Brugia malayi | |
| 1.1.1.49 | adenosine 5' [gamma-thio] triphosphate | - |
Brugia malayi | |
| 1.1.1.49 | ADP | 15% inhibition at 2 mM | Brugia malayi | |
| 1.1.1.49 | AMP | 5% inhibition at 2 mM | Brugia malayi | |
| 1.1.1.49 | ATP | 31% inhibition at 5 mM, non-competitive inhibition with respect to D-glucose 6-phosphate | Brugia malayi | |
| 1.1.1.49 | Cu2+ | - |
Brugia malayi | |
| 1.1.1.49 | guanosine hydrochloride | the enzyme shows a sharp loss in activity above 0.75 M, but is activated by low concentrations of 0.2 M of GdmCl | Brugia malayi | |
| 1.1.1.49 | K+ | - |
Brugia malayi | |
| 1.1.1.49 | Li+ | - |
Brugia malayi | |
| 1.1.1.49 | Na+ | - |
Brugia malayi | |
| 1.1.1.49 | NADPH | competitive inhibition with respect to NADP+ and D-glucose 6-phosphate | Brugia malayi | |
| 1.1.1.49 | phosphoenol pyruvate | 25% inhibition at 10 mM | Brugia malayi | |
| 1.1.1.49 | suramin | 77% inhibition at 0.05 mM | Brugia malayi | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.49 | additional information | - |
additional information | Michaelis-Menten kinetics | Brugia malayi |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | Mg2+ | required | Brugia malayi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 75000 | - |
4 * 75000, recombinant His-tagged enzyme, SDS-PAGE | Brugia malayi |
| 1.1.1.49 | 300000 | - |
recombinant His-tagged enzyme, gel filtration | Brugia malayi |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | Brugia malayi | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.49 | Brugia malayi | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.49 | recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography | Brugia malayi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.49 | D-glucose 6-phosphate + NADP+ | - |
Brugia malayi | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.49 | More | rBmG6PD is composed of 37% alpha-helices and 26% beta-sheets | Brugia malayi |
| 1.1.1.49 | tetramer | 4 * 75000, recombinant His-tagged enzyme, SDS-PAGE | Brugia malayi |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.49 | G6PD | - |
Brugia malayi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 40 | - |
- |
Brugia malayi |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 50 | - |
purified recombinant His-tagged enzyme, inactivation | Brugia malayi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.49 | 8.5 | - |
- |
Brugia malayi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.49 | NADP+ | - |
Brugia malayi | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.49 | 0.025 | - |
NADPH | versus D-glucose 6-phosphate, pH 8.0, 25°C | Brugia malayi | |
| 1.1.1.49 | 0.04 | - |
NADPH | versus NADP+, pH 8.0, 25°C | Brugia malayi | |
| 1.1.1.49 | 6.5 | - |
ATP | pH 8.0, 25°C | Brugia malayi | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.49 | metabolism | regulatory enzyme of the pentose phosphate pathway | Brugia malayi |
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