EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.164 | expression in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Crystallization (Comment) | Organism |
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2.7.1.164 | in complex with an anticodon-stem/loop truncated tRNASec, to 2.4 A resolution. Truncated tRNASec is bound between the enzyme's C-terminal domain CTD and N-terminal kinase domain NTD that are connected by a flexible 11 amino acid linker. Upon tRNASec recognition, the CTD undergoes a 62-A movement to allow proper binding of the 7-bp D-stem. This large reorganization of the quaternary structure likely provides a means by which the unique tRNASec species can be accurately recognized with high affinity by the translation machinery | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.164 | D146A | mutant is active in vivo | Methanocaldococcus jannaschii |
2.7.1.164 | K142A | mutant is active in vivo | Methanocaldococcus jannaschii |
2.7.1.164 | K142A/Y143A | mutant shows severely reduced activity with the Methanopyrus kandleri tRNASec substrate | Methanocaldococcus jannaschii |
2.7.1.164 | K17A | mutation abolishes catalytic activity and and inhibits tRNASec recognition | Methanocaldococcus jannaschii |
2.7.1.164 | K30A | mutant is defective in phosphorylation activity | Methanocaldococcus jannaschii |
2.7.1.164 | additional information | truncation of the C-termional domain. Deletions of up to 98 amino acids, PSTK1-153, still show activity, albeit at a much reduced level of about 6% of the initial velocity of the intact enzyme. In vivo, PSTK1-153 is still able compensate for the Escherichia coli selA deletion. Truncation PSTK1-192 lacks the entire CTD domain and exhibits 40% residual activity. PSTK1-215 and PSTK1-240 mutants lack the terminal two and one helices of the helix bundle, respectively. PSTK1-215 mutant shows remarkably reduced activity | Methanocaldococcus jannaschii |
2.7.1.164 | N161A | mutant is defective in phosphorylation activity | Methanocaldococcus jannaschii |
2.7.1.164 | S18A | mutation abolishes catalytic activity and and inhibits tRNASec recognition | Methanocaldococcus jannaschii |
2.7.1.164 | W145A | mutant is active in vivo | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.164 | Methanocaldococcus jannaschii | Q58933 | - |
- |
2.7.1.164 | Methanocaldococcus jannaschii DSM 2661 | Q58933 | - |
- |