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Literature summary extracted from

  • Witte, G.; Hartung, S.; Buettner, K.; Hopfner, K.P.
    Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates (2008), Mol. Cell, 30, 167-178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.85 expression in Escherichia coli Bacillus subtilis
2.7.7.85 expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.85 crystallisation of apoenzyme, apoenzyme + ATPgammaS, apoenzyme + cordycepin-triphosphate, determination of the crystal structure at 2.1 A Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.85 Mg2+
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.85 42700
-
 2 * 42700 Thermotoga maritima
2.7.7.85 42900
-
 8 * 42900 Bacillus subtilis
2.7.7.85 300000
-
 gel filtration Bacillus subtilis
2.7.7.85 353500
-
 analytical ultracentrifugation Thermotoga maritima
2.7.7.85 370000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.85 2 ATP Thermotoga maritima specific recognition of adenine in the active site pocked. The enzyme signals DNA structures that interfere with chromosome segregation cyclic di-3',5'-adenylate. The enzyme activity is unaffected by linear DNA or DNA ends but strongly suppressed by branched nucleic acids such as Holliday junctions 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.85 Bacillus subtilis P37573
-
-
2.7.7.85 Thermotoga maritima Q9WY43
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.85
-
 Bacillus subtilis
2.7.7.85
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.85 2 ATP specific recognition of adenine in the active site pocked. The enzyme signals DNA structures that interfere with chromosome segregation cyclic di-3',5'-adenylate. The enzyme activity is unaffected by linear DNA or DNA ends but strongly suppressed by branched nucleic acids such as Holliday junctions Thermotoga maritima 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?
2.7.7.85 2 ATP specific recognition of adenine in the active site pocked. The enzyme does not convert GTP to cyclic di-3',5'-guanylate Thermotoga maritima 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.85 octamer 2 * 42700 Thermotoga maritima
2.7.7.85 octamer 8 * 42900 Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.85 BsuDisA
-
 Bacillus subtilis
2.7.7.85 TmaDisA
-
 Thermotoga maritima

General Information

EC Number General Information Comment Organism
2.7.7.85 physiological function the enzyme signals DNA structures that interfere with chromosome segregation cyclic di-3',5'-adenylate. The enzyme activity is unaffected by linear DNA or DNA ends but strongly suppressed by branched nucleic acids such as Holliday junctions Thermotoga maritima