EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.16 | recombinant overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain SG13009 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.16 | C159A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Escherichia coli |
2.5.1.16 | D158A | site-directed mutagenesis, inactive mutant | Escherichia coli |
2.5.1.16 | D161A | site-directed mutagenesis, inactive mutant | Escherichia coli |
2.5.1.16 | I163A | site-directed mutagenesis, replacement of Ile163 has no influence on EcSDPS activity | Escherichia coli |
2.5.1.16 | P162A | site-directed mutagenesis, replacement of Pro162 has no influence on EcSDPS activity | Escherichia coli |
2.5.1.16 | P165Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Escherichia coli |
2.5.1.16 | T160A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.16 | 0.029 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant I163A | Escherichia coli | |
2.5.1.16 | 0.032 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant wild-type enzyme | Escherichia coli | |
2.5.1.16 | 0.033 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159S | Escherichia coli | |
2.5.1.16 | 0.035 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P162A | Escherichia coli | |
2.5.1.16 | 0.038 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159A | Escherichia coli | |
2.5.1.16 | 0.05 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant T160A | Escherichia coli | |
2.5.1.16 | 0.059 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P165A | Escherichia coli | |
2.5.1.16 | 0.0778 | - |
putrescine | pH 7.5, 37°C, recombinant wild-type enzyme | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 33252 | - |
2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
2.5.1.16 | 33823 | - |
2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
2.5.1.16 | 36500 | - |
2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.16 | S-adenosylmethioninamine + putrescine | Escherichia coli | - |
S-methyl-5'-thioadenosine + spermidine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.16 | Escherichia coli | P09158 | strain MG1655, gene speE | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.16 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain SG13009 | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.16 | S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | Escherichia coli SPDS follows the ping-pong mechanism, in which an aminopropylated enzyme intermediate was formed by the interaction of SPDS with dcSAM, prior to reaction with putrescine | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.16 | S-adenosylmethioninamine + putrescine | - |
Escherichia coli | S-methyl-5'-thioadenosine + spermidine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.16 | homodimer | 2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.16 | SPDS | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 50 | - |
- |
Escherichia coli |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 37 | 50 | sharp drop in activity above optimal temperature 50°C, profile overview | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.16 | 0.128 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159A | Escherichia coli | |
2.5.1.16 | 0.135 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant T160A | Escherichia coli | |
2.5.1.16 | 0.138 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant I163A | Escherichia coli | |
2.5.1.16 | 0.14 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant wild-type enzyme | Escherichia coli | |
2.5.1.16 | 0.145 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159S | Escherichia coli | |
2.5.1.16 | 0.15 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P165A | Escherichia coli | |
2.5.1.16 | 0.156 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P162A | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.16 | 7.5 | - |
- |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.16 | physiological function | spermidine synthase plays a crucial role in cell proliferation and differentiation | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.16 | 2.5 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P165A | Escherichia coli | |
2.5.1.16 | 2.7 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant T160A | Escherichia coli | |
2.5.1.16 | 3.4 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant C159A | Escherichia coli | |
2.5.1.16 | 4.4 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant wild-type enzyme and SPS mutant C159S | Escherichia coli | |
2.5.1.16 | 4.5 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant P162A | Escherichia coli | |
2.5.1.16 | 4.8 | - |
S-adenosylmethioninamine | pH 7.5, 37°C, recombinant SPS mutant I163A | Escherichia coli |