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Literature summary extracted from

  • Yoshida, K.; Sanbongi, A.; Murakami, A.; Suzuki, H.; Takenaka, S.; Takami, H.
    Three inositol dehydrogenases involved in utilization and interconversion of inositol stereoisomers in a thermophile, Geobacillus kaustophilus HTA426 (2012), Microbiology, 158, 1942-1952.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.18 organization of iol genes, three paralogous iol genes GK1897, GK1898, and GK1899 located in tandem within a large gene cluster, determination of the transcription start site, individual expression as His6-tagged proteins in Escherichia coli strain BL21(DE3) Geobacillus kaustophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.18 myo-inositol + NAD+ Geobacillus kaustophilus
-
 2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+
-
 r
1.1.1.18 myo-inositol + NAD+ Geobacillus kaustophilus PS8
-
 2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.18 Geobacillus kaustophilus
-
 three paralogous iol genes located in tandem within a large gene cluster, i.e. GK1897, GK1898, and GK1899
-
1.1.1.18 Geobacillus kaustophilus PS8
-
 three paralogous iol genes located in tandem within a large gene cluster, i.e. GK1897, GK1898, and GK1899
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.18 additional information Geobacillus kaustophilus strain HTA426 utilizes some inositol stereoisomers, including myo-, D-chiro- and scyllo-inositols, as sole carbon sources. Any of the three isozymes supports myo-inositol-dependent growth. GC-TOF-MS analysis of intracellular inositols in strain HTA426 Geobacillus kaustophilus
-
1.1.1.18 additional information Geobacillus kaustophilus strain PS8 utilizes myo-inositol as sole carbon source. Any of the three isozymes supports myo-inositol-dependent growth. GC-TOF-MS analysis of intracellular inositols in strain PS8 Geobacillus kaustophilus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.18 myo-inositol + NAD+
-
 Geobacillus kaustophilus 2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+
-
 r
1.1.1.18 myo-inositol + NAD+
-
 Geobacillus kaustophilus PS8 2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.18 40
-
 assay at Geobacillus kaustophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.18 8
-
 assay at Geobacillus kaustophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.18 NAD+
-
 Geobacillus kaustophilus
1.1.1.18 NADH
-
 Geobacillus kaustophilus

General Information

EC Number General Information Comment Organism
1.1.1.18 physiological function Geobacillus kaustophilus HTA426 utilizes some inositol stereoisomers, including myo-, D-chiro- and scyllo-inositols, as sole carbon sources. Any of the three isozymes supports MI-dependent growth Geobacillus kaustophilus