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Literature summary extracted from

  • Tsunasawa, S.; Nakura, S.; Tanigawa, T.; Kato, I.
    Pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Pyrococcus furiosus: cloning and overexpression in Escherichia coli of the gene, and its application to protein sequence analysis (1998), J. Biochem., 124, 778-783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.3 overexpression in Escherichia coli Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.19.3 C142S complete loss of the activity Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.3 Co2+
-
 Pyrococcus furiosus
3.4.19.3 Hg2+
-
 Pyrococcus furiosus
3.4.19.3 N-ethylmaleimide 1 mM, activity is completely lost Pyrococcus furiosus
3.4.19.3 p-chloromercuribenzoate 1 mM, activity is completely lost Pyrococcus furiosus
3.4.19.3 Zn2+
-
 Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.3 22936
-
 x * 22936, ionspray triple quadrupole mass spectrometry, carboxymethylated enzyme Pyrococcus furiosus
3.4.19.3 22940
-
 ionspray triple quadrupole mass spectrometry, carboxymethylated enzyme Pyrococcus furiosus
3.4.19.3 45640
-
 ionspray triple quadrupole mass spectrometry, native enzyme Pyrococcus furiosus
3.4.19.3 45643
-
 x * 45643, ionspray triple quadrupole mass spectrometry, native enzyme Pyrococcus furiosus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.19.3 guanidine-HCl approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min Pyrococcus furiosus
3.4.19.3 SDS approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min Pyrococcus furiosus
3.4.19.3 urea approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Pyrococcus furiosus O73944
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.3
-
 Pyrococcus furiosus

Subunits

EC Number Subunits Comment Organism
3.4.19.3 ? x * 22936, ionspray triple quadrupole mass spectrometry, carboxymethylated enzyme Pyrococcus furiosus
3.4.19.3 ? x * 45643, ionspray triple quadrupole mass spectrometry, native enzyme Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.4.19.3 PCP
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.3 90
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.19.3 75 105 75°C: about 60% of maximal activity, 105°C: about 90% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.3 75
-
 150 min, more than 80% of the activity is retained Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.3 7 9
-
 Pyrococcus furiosus