Literature summary extracted from

Tasi, Y.C.; Chao, H.C.; Chung, C.L.; Liu, X.Y.; Lin, Y.M.; Liao, P.C.; Pan, H.A.; Chiang, H.S.; Kuo, P.L.; Lin, Y.H.
Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker (2013), J. Assist. Reprod. Genet., 30, 505-512.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.1.1.31	mitochondrion	-	Homo sapiens	5739	-
1.1.1.31	additional information	the enzyme is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm	Homo sapiens	-	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.31	3-hydroxy-2-methylpropanoate + NAD+	Homo sapiens	-	2-methyl-3-oxopropanoate + NADH + H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.31	Homo sapiens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.31	cerebellum	-	Homo sapiens	-
1.1.1.31	heart	-	Homo sapiens	-
1.1.1.31	kidney	-	Homo sapiens	-
1.1.1.31	liver	-	Homo sapiens	-
1.1.1.31	additional information	HIBADH is downregulated in low-motility sperm, and is enriched expressed during human spermiogenesis, quantitative RT-PCR expression analysis, overview	Homo sapiens	-
1.1.1.31	neuronal cell	-	Homo sapiens	-
1.1.1.31	placenta	-	Homo sapiens	-
1.1.1.31	skeletal muscle	-	Homo sapiens	-
1.1.1.31	sperm	elongating, elongated, and mature sperm	Homo sapiens	-
1.1.1.31	testis	-	Homo sapiens	-
1.1.1.31	uterus	-	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.31	3-hydroxy-2-methylpropanoate + NAD+	-	Homo sapiens	2-methyl-3-oxopropanoate + NADH + H+	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.31	HIBADH	-	Homo sapiens

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.31	20	-	assay at	Homo sapiens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.31	10	-	assay at	Homo sapiens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.31	NAD+	-	Homo sapiens

Expression

EC Number	Organism	Comment	Expression
1.1.1.31	Homo sapiens	HIBADH is downregulated in low-motility sperm, and is enriched expressed during human spermiogenesis	down
1.1.1.31	Homo sapiens	HIBADH is downregulated in low-motility sperm, and is enriched expressed during human spermiogenesis	up

General Information

EC Number	General Information	Comment	Organism
1.1.1.31	evolution	3-hydroxyisobutyrate dehydrogenase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donors with NAD+ or NADP+ acceptors	Homo sapiens
1.1.1.31	additional information	enzyme activity of HIBADH in spermatozoa from patients with asthenozoospermia is reduced compared to controls	Homo sapiens
1.1.1.31	physiological function	3-hydroxyisobutyrate dehydrogenase is one of the critical enzymes generating glucose by metabolizing amino acids in the gluconeogenesis pathway. It participates in valine, leucine, and isoleucine degradation and is a central metabolic enzyme in the valine catabolic pathway. The enzyme plays an important role in regulating sperm motility and may serve as a novel sperm-motility marker	Homo sapiens

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Release 2023.1 (January 2023)