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Literature summary extracted from

  • Petzelbauer, I.; Reiter, A.; Splechtna, B.; Kosma, P.; Nidetzky, B.
    Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make major contributions to the formation of new beta-glycosides (2000), Eur. J. Biochem., 267, 5055-5066.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 1.1
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B26 192
-
 methyl beta-D-galactoside release of methanol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B26 196
-
 lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B28 5.3
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B28 186
-
 lactose release of D-glucose, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B28 861
-
 methyl beta-D-galactoside release of methanol, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B34 1.1
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B34 192
-
 methyl beta-D-galactoside relase, of methanol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B34 196
-
 lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus P22498
-
-
3.2.1.B26 Saccharolobus solfataricus DSM 1617 P22498
-
-
3.2.1.B28 Pyrococcus furiosus Q51723
-
-
3.2.1.B34 Saccharolobus solfataricus P22498
-
-
3.2.1.B34 Saccharolobus solfataricus DSM 1617 P22498
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 2-nitrophenyl beta-D-galactoside
-
 Saccharolobus solfataricus 2-nitrophenol + D-galactose
-
 r
3.2.1.B26 2-nitrophenyl beta-D-galactoside
-
 Saccharolobus solfataricus DSM 1617 2-nitrophenol + D-galactose
-
 r
3.2.1.B26 lactose + H2O
-
 Saccharolobus solfataricus D-glucose + D-galactose
-
 r
3.2.1.B26 lactose + H2O
-
 Saccharolobus solfataricus DSM 1617 D-glucose + D-galactose
-
 r
3.2.1.B26 methyl beta-D-galactoside
-
 Saccharolobus solfataricus methanol + D-galactose
-
 r
3.2.1.B26 methyl beta-D-galactoside
-
 Saccharolobus solfataricus DSM 1617 methanol + D-galactose
-
 r
3.2.1.B26 additional information enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose Saccharolobus solfataricus ?
-
 ?
3.2.1.B26 additional information enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose Saccharolobus solfataricus DSM 1617 ?
-
 ?
3.2.1.B28 2-nitrophenyl beta-D-galactoside
-
 Pyrococcus furiosus 2-nitrophenol + D-galactose
-
 r
3.2.1.B28 lactose + H2O
-
 Pyrococcus furiosus D-glucose + D-galactose
-
 r
3.2.1.B28 methyl beta-D-galactoside
-
 Pyrococcus furiosus methanol + D-galactose
-
 r
3.2.1.B28 additional information enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose Pyrococcus furiosus ?
-
 ?
3.2.1.B34 2-nitrophenyl beta-D-galactoside
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
 r
3.2.1.B34 lactose + H2O
-
 Saccharolobus solfataricus D-glucose + D-galactose
-
 r
3.2.1.B34 methyl beta-D-galactoside
-
 Saccharolobus solfataricus methanol + beta-D-galactose
-
 r
3.2.1.B34 additional information enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose Saccharolobus solfataricus ?
-
 ?
3.2.1.B34 additional information enzyme additionally catalyzes transglycosylation reactions, making new beta(1->3) and beta(1->6) glycosidic bonds by intermolecular as well as intramolecular transfer reactions. The intramolecular galactosyl transfer of CelB yields beta-D-Galp-(1->6)-D-glucose and beta-D-Galp-(1->3)-D-glucose in a molar ratio of about 1 : 2. Galactosyl transfer from CelB to D-glucose occurs with partitioning ratios, kNu /kwater, which are 170 times those for the reactions of the galactosylated enzyme with 2-propanol. Therefore, the binding interactions with nucleophiles contribute chiefly to formation of new beta-glycosides during lactose conversion. Likewise, noncovalent interactions with the glucose leaving group govern the catalytic efficiencies for the hydrolysis of lactose Saccharolobus solfataricus DSM 1617 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 LACS
-
 Saccharolobus solfataricus
3.2.1.B34 LACS
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 6.6
-
 methyl beta-D-galactoside release of methanol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B26 1300
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B26 1500
-
 lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B28 8.3
-
 methyl beta-D-galactoside release of methanol, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B28 3800
-
 lactose release of D-glucose, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B28 10000
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B34 6.6
-
 methyl beta-D-galactoside relase, of methanol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B34 1300
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B34 1500
-
 lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.23 7.7
-
 lactose pH 6.5, 80°C Saccharolobus solfataricus
3.2.1.23 20.4
-
 lactose pH 6.5, 80°C Pyrococcus furiosus
3.2.1.23 1200
-
 2-nitrophenyl beta-D-galactoside pH 6.5, 80°C Saccharolobus solfataricus
3.2.1.23 1900
-
 2-nitrophenyl beta-D-galactoside pH 6.5, 80°C Pyrococcus furiosus
3.2.1.B26 0.034
-
 methyl beta-D-galactoside release of methanol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B26 7.7
-
 lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B26 1200
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B28 0.01
-
 methyl beta-D-galactoside release of methanol, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B28 20.4
-
 lactose release of D-glucose, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B28 1900
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Pyrococcus furiosus
3.2.1.B34 0.034
-
 methyl beta-D-galactoside relase, of methanol, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B34 7.7
-
 lactose release of D-glucose, pH 7.5, 80°C Saccharolobus solfataricus
3.2.1.B34 1200
-
 2-nitrophenyl beta-D-galactoside release of 2-nitrophenol, pH 7.5, 80°C Saccharolobus solfataricus