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Literature summary extracted from

  • Oppermann, U.C.; Maser, E.
    Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni (1996), Eur. J. Biochem., 241, 744-749.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.357 0.003
-
 Fusidic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
1.1.1.357 0.0068
-
 Fusidic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
1.1.1.357 0.0111
-
 5beta-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 0.0152
-
 3alpha-hydroxydesogestrel pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
1.1.1.357 0.0161
-
 5alpha-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 0.019
-
 5alpha-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1.1.1.357 0.0201
-
 5beta-dihydrotestosterone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1.1.1.357 0.0207
-
 epiandrosterone pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
1.1.1.357 0.0216
-
 3alpha-hydroxydesogestrel pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
1.1.1.357 0.0244
-
 androsterone pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
1.1.1.357 0.025
-
 3-dehydro-alpha-ecdysone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 0.025
-
 cholic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
1.1.1.357 0.0267
-
 androsterone pH 8.9, temperature not specified in the publication, NAD(P)+ Pseudomonas sp.
1.1.1.357 0.028
-
 3-dehydro-alpha-ecdysone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1.1.1.357 0.0301
-
 cholic acid pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
1.1.1.357 0.0328
-
 androstandiol pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni
1.1.1.357 0.035
-
 3-dehydro-beta-ecdysone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 0.0357
-
 androstandione pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 0.61
-
 Metyrapone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 0.63
-
 Metyrapone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1.1.1.357 0.9
-
 4-Nitroacetophenone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 1
-
 4-nitrobenzaldehyde pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1.1.1.357 1
-
 4-Nitroacetophenone pH 7.0, temperature not specified in the publication, NAD(P)H Pseudomonas sp.
1.1.1.357 1
-
 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 1.09
-
 4-nitrobenzaldehyde pH 7.0, temperature not specified in the publication, NAD(P)H Comamonas testosteroni
1.1.1.357 2.1
-
 metyrapol pH 8.9, temperature not specified in the publication, NAD(P)+ Comamonas testosteroni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.357 cytoplasm
-
 Comamonas testosteroni 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.357 28000
-
 SDS-PAGE Comamonas testosteroni
1.1.1.357 28000
-
 SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.357 a 3alpha-hydroxysteroid + NAD(P)+ Comamonas testosteroni
-
 a 3-oxosteroid + NAD(P)H + H+
-
 ?
1.1.1.357 a 3alpha-hydroxysteroid + NAD(P)+ Pseudomonas sp.
-
 a 3-oxosteroid + NAD(P)H + H+
-
 ?
1.1.1.357 a 3alpha-hydroxysteroid + NAD(P)+ Comamonas testosteroni ATCC 11996
-
 a 3-oxosteroid + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.357 Comamonas testosteroni P80702
-
-
1.1.1.357 Comamonas testosteroni ATCC 11996 P80702
-
-
1.1.1.357 Pseudomonas sp. P80701 Boehringer patent strain
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.357 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone + NAD(P)H + H+ 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone i.e. NKI 42255 Comamonas testosteroni 2-(1H-imidazol-1-yl)-1-(4-methoxyphenyl)-2-methylpropan-1-ol + NAD(P)+
-
 ?
1.1.1.357 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone + NAD(P)H + H+ 2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-propanone i.e. NKI 42255 Comamonas testosteroni ATCC 11996 2-(1H-imidazol-1-yl)-1-(4-methoxyphenyl)-2-methylpropan-1-ol + NAD(P)+
-
 ?
1.1.1.357 3-dehydro-alpha-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni alpha-ecdysone + NAD(P)+
-
 ?
1.1.1.357 3-dehydro-alpha-ecdysone + NAD(P)H + H+
-
 Pseudomonas sp. alpha-ecdysone + NAD(P)+
-
 ?
1.1.1.357 3-dehydro-alpha-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni ATCC 11996 alpha-ecdysone + NAD(P)+
-
 ?
1.1.1.357 3-dehydro-beta-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni beta-ecdysone + NAD(P)+
-
 ?
1.1.1.357 3-dehydro-beta-ecdysone + NAD(P)H + H+
-
 Comamonas testosteroni ATCC 11996 beta-ecdysone + NAD(P)+
-
 ?
1.1.1.357 3alpha-hydroxydesogestrel + NAD(P)+
-
 Comamonas testosteroni 3-oxodesogestrel + NAD(P)H + H+
-
 ?
1.1.1.357 3alpha-hydroxydesogestrel + NAD(P)+
-
 Pseudomonas sp. 3-oxodesogestrel + NAD(P)H + H+
-
 ?
1.1.1.357 4-nitroacetophenone + NAD(P)H + H+
-
 Comamonas testosteroni 1-(4-nitrophenyl)ethanol + NAD(P)+
-
 ?
1.1.1.357 4-nitroacetophenone + NAD(P)H + H+
-
 Pseudomonas sp. 1-(4-nitrophenyl)ethanol + NAD(P)+
-
 ?
1.1.1.357 4-nitrobenzaldehyde + NAD(P)H + H+
-
 Comamonas testosteroni (4-nitrophenyl)methanol + NAD(P)+
-
 ?
1.1.1.357 4-nitrobenzaldehyde + NAD(P)H + H+
-
 Pseudomonas sp. (4-nitrophenyl)methanol + NAD(P)+
-
 ?
1.1.1.357 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Comamonas testosteroni (5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
1.1.1.357 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Pseudomonas sp. (5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
1.1.1.357 5beta-dihydrotestosterone + NAD(P)H + H+
-
 Comamonas testosteroni (5beta,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
1.1.1.357 5beta-dihydrotestosterone + NAD(P)H + H+
-
 Pseudomonas sp. (5beta,17beta)-androstane-3,17-diol + NAD(P)+
-
 ?
1.1.1.357 a 3alpha-hydroxysteroid + NAD(P)+
-
 Comamonas testosteroni a 3-oxosteroid + NAD(P)H + H+
-
 ?
1.1.1.357 a 3alpha-hydroxysteroid + NAD(P)+
-
 Pseudomonas sp. a 3-oxosteroid + NAD(P)H + H+
-
 ?
1.1.1.357 a 3alpha-hydroxysteroid + NAD(P)+
-
 Comamonas testosteroni ATCC 11996 a 3-oxosteroid + NAD(P)H + H+
-
 ?
1.1.1.357 androstandiol + NAD(P)+
-
 Comamonas testosteroni (5alpha,17beta)-17-hydroxyandrostan-3-one + NAD(P)H + H+
-
 ?
1.1.1.357 androstandione + NAD(P)H + H+
-
 Comamonas testosteroni 3-hydroxyandrostan-17-one + NAD(P)+
-
 ?
1.1.1.357 androsterone + NAD(P)+
-
 Comamonas testosteroni (5alpha)-androstane-3,17-dione + NAD(P)H + H+
-
 ?
1.1.1.357 androsterone + NAD(P)+
-
 Pseudomonas sp. (5alpha)-androstane-3,17-dione + NAD(P)H + H+
-
 ?
1.1.1.357 cholic acid + NAD(P)+
-
 Comamonas testosteroni (5beta,7alpha,8xi,12alpha)-7,12-dihydroxy-3-oxocholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.357 cholic acid + NAD(P)+
-
 Pseudomonas sp. (5beta,7alpha,8xi,12alpha)-7,12-dihydroxy-3-oxocholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.357 epiandrosterone + NAD(P)+
-
 Comamonas testosteroni (5alpha)-androstane-3,17-dione + NAD(P)H + H+
-
 ?
1.1.1.357 fusidic acid + NAD(P)+
-
 Comamonas testosteroni (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NAD(P)H + H+
-
 ?
1.1.1.357 fusidic acid + NAD(P)+
-
 Pseudomonas sp. (2Z)-2-[(4alpha,5alpha,8alpha,9beta,11alpha,13alpha,14beta,16beta,17Z)-16-(acetyloxy)-11-hydroxy-4,8,10,14-tetramethyl-3-oxogonan-17-ylidene]-6-methylhept-5-enoic acid + NAD(P)H + H+
-
 ?
1.1.1.357 metyrapol + NAD(P)+
-
 Comamonas testosteroni 2-methyl-1,2-di(pyridin-3-yl)propan-1-one + NAD(P)H + H+
-
 ?
1.1.1.357 metyrapone + NAD(P)H + H+
-
 Comamonas testosteroni 2-methyl-1,2-di(pyridin-3-yl)propan-1-ol + NAD(P)+
-
 ?
1.1.1.357 metyrapone + NAD(P)H + H+
-
 Pseudomonas sp. 2-methyl-1,2-di(pyridin-3-yl)propan-1-ol + NAD(P)+
-
 ?
1.1.1.357 additional information no activity with testosterone, 3-oxo-desogestrel, progesterone, 4-nitrobenzalcohol, trans-benzene dihydrodiol and cis-benzene dihydrodiol Comamonas testosteroni ?
-
 ?
1.1.1.357 additional information no activity with testosterone, 3-oxo-desogestrel, progesterone, NKI 42255, trans-benzene dihydrodiol and cis-benzene dihydrodiol Pseudomonas sp. ?
-
 ?
1.1.1.357 additional information no activity with testosterone, 3-oxo-desogestrel, progesterone, 4-nitrobenzalcohol, trans-benzene dihydrodiol and cis-benzene dihydrodiol Comamonas testosteroni ATCC 11996 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.357 monomer 1 * 28000, SDS-PAGE Comamonas testosteroni
1.1.1.357 monomer 1 * 28000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.357 HSD 28
-
 Comamonas testosteroni
1.1.1.357 HSD 29
-
 Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.1.1.357 metabolism enzyme is involved in inactivation of steroid hormones Comamonas testosteroni
1.1.1.357 metabolism enzyme is involved in inactivation of steroid hormones Pseudomonas sp.