EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.55 | the X-ray structures, determined for the mutants with altered KANRS motif, indicate no gross structural penalty resulting from mutation, but the subtle changes observed in the active sites of these mutant proteins correlate with their altered catalytic function | Neisseria meningitidis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.55 | A56P/N57DELTA | mutation in the absolutely conserved KANRS motif. Complete loss of activity | Acidithiobacillus ferrooxidans |
2.5.1.55 | A56P/N57DELTA | mutation in the absolutely conserved KANRS motif. Complete loss of activity | Neisseria meningitidis |
2.5.1.55 | K55A | mutation in the absolutely conserved KANRS motif. Complete loss of activity | Acidithiobacillus ferrooxidans |
2.5.1.55 | K57A | mutation in the absolutely conserved KANRS motif. Complete loss of activity | Neisseria meningitidis |
2.5.1.55 | N57A | mutation in the absolutely conserved KANRS motif. About 2% of wild-type activity | Acidithiobacillus ferrooxidans |
2.5.1.55 | N57D | mutation in the absolutely conserved KANRS motif. Complete loss of activity | Neisseria meningitidis |
2.5.1.55 | N57DELTA | mutation in the absolutely conserved KANRS motif. Complete loss of activity | Acidithiobacillus ferrooxidans |
2.5.1.55 | N59A | mutation in the absolutely conserved KANRS motif. About 0.2% of wild-type activity | Neisseria meningitidis |
2.5.1.55 | N59DELTA | mutation in the absolutely conserved KANRS motif. Less than 0.1% of wild-type activity | Neisseria meningitidis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.55 | 0.0025 | - |
D-arabinose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.0029 | - |
phosphoenolpyruvate | mutant N57A, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.0079 | - |
D-arabinose 5-phosphate | mutant N59A, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.012 | - |
phosphoenolpyruvate | wild-type, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.012 | - |
D-arabinose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.022 | - |
D-arabinose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.043 | - |
D-arabinose 5-phosphate | mutant N59DELTA, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.081 | - |
D-arabinose 5-phosphate | mutant N57A, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.23 | - |
2-deoxy-D-ribose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.432 | - |
2-deoxy-D-ribose 5-phosphate | mutant N59A, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.503 | - |
D-arabinose 5-phosphate | mutant N59A, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 1.1 | - |
D-arabinose 5-phosphate | mutant N59DELTA, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 1.5 | - |
2-deoxy-D-ribose 5-phosphate | mutant N57A, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 5 | - |
2-deoxy-D-ribose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.55 | Acidithiobacillus ferrooxidans | - |
- |
- |
2.5.1.55 | Neisseria meningitidis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.55 | phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate | - |
Acidithiobacillus ferrooxidans | ? + phosphate | - |
? | |
2.5.1.55 | phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate | - |
Neisseria meningitidis | ? + phosphate | - |
? | |
2.5.1.55 | phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Acidithiobacillus ferrooxidans | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? | |
2.5.1.55 | phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Neisseria meningitidis | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.55 | 0.007 | - |
2-deoxy-D-ribose 5-phosphate | mutant N59A, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.13 | - |
2-deoxy-D-ribose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.149 | - |
D-arabinose 5-phosphate | mutant N59A, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.178 | - |
D-arabinose 5-phosphate | mutant N59DELTA, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.202 | - |
2-deoxy-D-ribose 5-phosphate | mutant N57A, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.3 | - |
D-arabinose 5-phosphate | mutant N57A, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.66 | - |
2-deoxy-D-ribose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 4.8 | - |
D-arabinose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 8 | - |
D-arabinose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Neisseria meningitidis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.55 | 0.016 | - |
2-deoxy-D-ribose 5-phosphate | mutant N59A, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.016 | - |
D-arabinose 5-phosphate | mutant N59DELTA, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.13 | - |
2-deoxy-D-ribose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.13 | - |
2-deoxy-D-ribose 5-phosphate | mutant N57A, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 0.29 | - |
D-arabinose 5-phosphate | mutant N59A, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 0.57 | - |
2-deoxy-D-ribose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Neisseria meningitidis | |
2.5.1.55 | 3.7 | - |
D-arabinose 5-phosphate | mutant N57A, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 220 | - |
D-arabinose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Acidithiobacillus ferrooxidans | |
2.5.1.55 | 660 | - |
D-arabinose 5-phosphate | wild-type, pH 7.5, temperature not specified in the publication | Neisseria meningitidis |