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Literature summary extracted from

  • Suwannarangsee, S.; Kim, S.; Kim, O.C.; Oh, D.B.; Seo, J.W.; Kim, C.H.; Rhee, S.K.; Kang, H.A.; Chulalaksananukul, W.; Kwon, O.
    Characterization of alcohol dehydrogenase 3 of the thermotolerant methylotrophic yeast Hansenula polymorpha (2012), Appl. Microbiol. Biotechnol., 96, 697-709.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 gene ADH3, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged enzyme in Echerichia coli strain BL21 (DE3), complementation of the HpADH3 mutant by an HpADH3 expression cassette fused to a strong constitutive promoter, the resulting strain produced a significantly increased amount of ethanol compared to the wild-type strain in a glucose medium, while in a xylose medium, the ethanol production is dramatically reduced in an HpADH3 overproduction strain compared to that in the wild-type strain, semi-quantitative RT-PCR analysis Ogataea angusta

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.1 additional information generation of the ADH3 deletion mutant, complementation of the HpADH3 mutant by an HpADH3 expression cassette fused to a strong constitutive promoter, the resulting strain produced a significantly increased amount of ethanol compared to the wild-type strain in a glucose medium, while in a xylose medium, the ethanol production is dramatically reduced in an HpADH3 overproduction strain compared to that in the wild-type strain, phenotype analysis of DELTAHpADH3 and DELTAHpADH1/DELTAHpADH3 mutants, overview Ogataea angusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.45
-
 ethanol pH 9.0, 22°C, recombinant enzyme Ogataea angusta
1.1.1.1 0.67
-
 acetaldehyde pH 6.0, 22°C, recombinant enzyme Ogataea angusta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.1 mitochondrion HpADH3 Ogataea angusta 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.1 acetaldehyde + NADH + H+ Ogataea angusta
-
 ethanol + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+ Ogataea angusta DL-1
-
 ethanol + NAD+
-
 r
1.1.1.1 ethanol + NAD+ Ogataea angusta
-
 acetaldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Ogataea angusta H9ZGN0 i.e. Pichia angusta, HpADH3 is not the major ADH in strain DL-1, gene ADH3
-
1.1.1.1 Ogataea angusta DL-1 H9ZGN0 i.e. Pichia angusta, HpADH3 is not the major ADH in strain DL-1, gene ADH3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 recombinant His6-tagged enzyme from Echerichia coli strain BL21 (DE3) by nickel affinity chromatography Ogataea angusta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1,2-butanediol + NAD+ low activity Ogataea angusta ?
-
 ?
1.1.1.1 1,2-butanediol + NAD+ low activity Ogataea angusta DL-1 ?
-
 ?
1.1.1.1 1-butanol + NAD+
-
 Ogataea angusta butanal + NADH + H+
-
 r
1.1.1.1 1-butanol + NAD+
-
 Ogataea angusta DL-1 butanal + NADH + H+
-
 r
1.1.1.1 1-octanol + NAD+
-
 Ogataea angusta octanal + NADH + H+
-
 r
1.1.1.1 1-pentanol + NAD+
-
 Ogataea angusta pentanal + NADH + H+
-
 r
1.1.1.1 1-propanol + NAD+
-
 Ogataea angusta propanaldehyde + NADH + H+
-
 r
1.1.1.1 2-methoxyethanol + NAD+
-
 Ogataea angusta 2-methoxyacetaldehyde + NADH + H+
-
 r
1.1.1.1 2-octanol + NAD+ low activity Ogataea angusta 2-octanone + NADH + H+
-
 r
1.1.1.1 2-pentanol + NAD+ low activity Ogataea angusta 2-pentanone + NADH + H+
-
 r
1.1.1.1 2-propanol + NAD+
-
 Ogataea angusta 2-propanone + NADH + H+
-
 r
1.1.1.1 acetaldehyde + NADH + H+
-
 Ogataea angusta ethanol + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+ best substrate Ogataea angusta ethanol + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+
-
 Ogataea angusta DL-1 ethanol + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+ best substrate Ogataea angusta DL-1 ethanol + NAD+
-
 r
1.1.1.1 ethanol + NAD+
-
 Ogataea angusta acetaldehyde + NADH + H+
-
 r
1.1.1.1 additional information methanol, formaldehyde, and acetone are no substrates for HpADH3 Ogataea angusta ?
-
 ?
1.1.1.1 additional information methanol, formaldehyde, and acetone are no substrates for HpADH3 Ogataea angusta DL-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 alcohol dehydrogenase 3
-
 Ogataea angusta
1.1.1.1 HpADH3
-
 Ogataea angusta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 22
-
 assay at room temperature Ogataea angusta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 3000
-
 ethanol pH 9.0, 22°C, recombinant enzyme Ogataea angusta
1.1.1.1 3500
-
 acetaldehyde pH 6.0, 22°C, recombinant enzyme Ogataea angusta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6
-
 aldehyde reduction Ogataea angusta
1.1.1.1 9
-
 alcohol oxidation Ogataea angusta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Ogataea angusta
1.1.1.1 NADH
-
 Ogataea angusta

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 6667
-
 ethanol pH 9.0, 22°C, recombinant enzyme Ogataea angusta
1.1.1.1 70000
-
 acetaldehyde pH 6.0, 22°C, recombinant enzyme Ogataea angusta