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Literature summary extracted from

  • Stammen, S.; Schuller, F.; Dietrich, S.; Gamer, M.; Biedendieck, R.; Jahn, D.
    Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium (2010), Appl. Microbiol. Biotechnol., 88, 529-539.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.6 spermidine
-
 Vectrevirus K1E

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 gene k1ep, expression of His6-tagged K1E in Escherichia coli strain bL21(DE3), recombinant expression of K1E RNA polymerase in Bacillus megaterium strain MS941, a nprM deletion mutant of DSM319, using K1E promoters or SP6 promoter and a PEG-mediated transformation, method development and evaluation. The T7 RNAP promoter is not recognized at all Vectrevirus K1E

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+ essentially required, best at 10 mM Vectrevirus K1E

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 100000
-
 1 * 100000, SDS-PAGE Vectrevirus K1E

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Vectrevirus K1E Q2WC24 gene k1ep
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 recombinant His6-tagged K1E from Escherichia coli strain BL21(DE3) by nickel affinity chromatgraphy, dialysis and ultrafiltration Vectrevirus K1E

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 4600
-
 purified recombinant His6-tagged enzyme, pH 7.9, 37°C Vectrevirus K1E

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 rNTP + RNAn
-
 Vectrevirus K1E diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 monomer 1 * 100000, SDS-PAGE Vectrevirus K1E

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 DNA-dependent RNA polymerase
-
 Vectrevirus K1E
2.7.7.6 DNA-dependent RNAP
-
 Vectrevirus K1E
2.7.7.6 K1E RNAP
-
 Vectrevirus K1E
2.7.7.6 RNAP
-
 Vectrevirus K1E

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 37 40
-
 Vectrevirus K1E

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.6 25 50 activity range, profile, overview Vectrevirus K1E

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.6 25
-
 half-life time of K1E RNAP stored in buffer containing 20 mM Na3PO4, 100 mM NaCl, pH 7.7, 1 mM EDTA, 10 mM DTT, and 0.1% Triton X-100, supplemented with 50% glycerol, is 87 h Vectrevirus K1E
2.7.7.6 50
-
 half-life time of the K1E RNAP is 16.5 sec Vectrevirus K1E

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 7.9
-
-
 Vectrevirus K1E

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.6 7.5 9 80% of maximall activity within this range Vectrevirus K1E