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Literature summary extracted from

  • Yoon, J.Y.; Park, C.R.; Lee, H.H.; Suh, S.W.
    Overexpression, crystallization and preliminary X-ray crystallographic analysis of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii (2012), Acta Crystallogr. Sect. F, 68, 440-442.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6 expressed in Escherichia coli Rosetta2 (DE3) pLysS Pyrococcus horikoshii
4.3.3.6 overexpression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.6 crystallization at 22°C using 2-methyl-2,4-pentanediol as a precipitant. Crystals of PdxS diffract to 2.61 A resolution and belong to the monoclinic space group P2(1), with unit-cell parameters a = 59.30, b = 178.56, c = 109.23 A, beta = 102.97°. The asymmetric unit contained six monomers Pyrococcus horikoshii
4.3.3.6 hanging-drop vapour-diffusion method, 296 K, 2-methyl-2,4-pentanediol Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.6 37014
-
 6 * 37014, calculated, dynamic light-scattering analysis Pyrococcus horikoshii
4.3.3.6 200000
-
 dynamic light-scattering analysis Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Pyrococcus horikoshii
-
-
-
4.3.3.6 Pyrococcus horikoshii O59080
-
-
4.3.3.6 Pyrococcus horikoshii OT-3 O59080
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.6 ion exchange chromatography (Q Sepharose), gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 ribulose 5-phosphate + glyceraldehyde 3-phosphate + NH3
-
 Pyrococcus horikoshii pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.6 homohexamer 6 * 37014, calculated, dynamic light-scattering analysis Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
4.3.3.6 PdxS
-
 Pyrococcus horikoshii
4.3.3.6 PdxS PdxS catalyses the condensation of ribulose 5-phosphate, glyceraldehyde 3-phosphate and ammonia, while glutamine amidotransferase (PdxT) catalyses the production of ammonia from glutamine. PdxS and PdxT form a complex Pyrococcus horikoshii
4.3.3.6 Ph1355
-
 Pyrococcus horikoshii
4.3.3.6 pyridoxal biosynthesis lyase
-
 Pyrococcus horikoshii