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Literature summary extracted from

  • Zhang, H.; Damude, H.G.; Yadav, N.S.
    Three diacylglycerol acyltransferases contribute to oil biosynthesis and normal growth in Yarrowia lipolytica (2012), Yeast, 29, 25-38.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.158 gene pdat or YALI0E16797g, DNA and amino acid sequence determination and analysis. In vivo expression of DGAT1, DGAT2 and PDAT under the heterologous Yarrowia lipolytica YAT promoter results in 598%, 702% and 278% increases in total fatty acid % dry cell weight over the empty vector control Yarrowia lipolytica

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.158 additional information construction of pdat, diacylglycerol O-acyltransferases (EC 2.3.1.20) dgat1 and dgat2 single mutants, of dgat1/dgat2 double mutant, and of a dgat1/dgat2/pdat triple mutant, which show 70%, 57%, 36%, 18% and 13%, respectively, of wild-type total lipid% per dry cell weight, phenotypes, overview Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Yarrowia lipolytica Q6C3R2 isoform DGAT2
-
2.3.1.20 Yarrowia lipolytica Q6C9V5 isoform DGAT1
-
2.3.1.20 Yarrowia lipolytica ATCC 20362 Q6C3R2 isoform DGAT2
-
2.3.1.20 Yarrowia lipolytica ATCC 20362 Q6C9V5 isoform DGAT1
-
2.3.1.23 Yarrowia lipolytica Q6C5M4
-
-
2.3.1.23 Yarrowia lipolytica ATCC 20362 Q6C5M4
-
-
2.3.1.158 Yarrowia lipolytica Q6C5M4 gene pdat
-
2.3.1.158 Yarrowia lipolytica ATCC 20362 Q6C5M4 gene pdat
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 YALI0D07986g ORF name Yarrowia lipolytica
2.3.1.20 YALI0E32769g ORF name Yarrowia lipolytica
2.3.1.23 PGAT
-
 Yarrowia lipolytica
2.3.1.23 YALI0E16797g ORF name Yarrowia lipolytica
2.3.1.158 acyl-CoA-independent phospholipid:diacylglycerol acyltransferase
-
 Yarrowia lipolytica
2.3.1.158 PDAT
-
 Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.158 25
-
 assay at Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.158 7.2
-
 assay at Yarrowia lipolytica

General Information

EC Number General Information Comment Organism
2.3.1.20 physiological function in isoforms dgat1, dgat2, dgat1/dgat2 double mutant and dgat1/dgat2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 57%, 36%, 18% and 13%, respectively Yarrowia lipolytica
2.3.1.23 physiological function in a diacylglycerol acyltransferase 1/diacylglycerol acyltransferase 2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 13% Yarrowia lipolytica