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Literature summary extracted from

  • Bleeker, P.M.; Mirabella, R.; Diergaarde, P.J.; Vandoorn, A.; Tissier, A.; Kant, M.R.; Prins, M.; de Vos, M.
    Haring, M.A. and Schuurink, R.C. Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative (2012), Proc. Natl. Acad. Sci. USA, 109, 20124-20129.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.3.142 agriculture construction of an interspecific cross between Solanum lycopersicum cv Moneymaker and the wild tomato Solanum habrochaites PI127826 leads to production of 7-epizingiberene in a range of concentrations up to PI127826 levels in the F2 plants. Plants also accumulate monoterpenes and/or other sesquiterpenes at high concentrations. Data show a correlation between the concentration of 7-epizingiberene on the leaf surface and the defense against whiteflies. Stable expression of short chain cis-prenyltransferase zFPS and 7-epizingiberene synthase in Solanum lycopersicum results in accumulation of 7-epizingiberene. The production of 7-epizingiberene has no repellent effect on spider mites in a choice assay, but has a strong effect on their fecundity. Mites placed on leaf disks of transgenic plants exhibit a 40% higher mortality and, after 4 d, an 81% reduction in the number of eggs Solanum habrochaites

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.142 0.007
-
 (2Z,6Z)-farnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Solanum habrochaites

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.142 plastid
-
 Solanum habrochaites 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.142 84000
-
 x * 84000, calculated Solanum habrochaites

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.142 Solanum habrochaites
-
-
-
4.2.3.142 Solanum habrochaites PI127826
-
-
-
4.2.3.142 Solanum lycopersicum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.142 trichome
-
 Solanum habrochaites
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.142 (2Z,6Z)-farnesyl diphosphate exclusively uses (2Z,6Z)-farnesyl-diphosphate Solanum habrochaites 7-epizingiberene + diphosphate plus trace amounts of beta-sesquiphellandrene and curcumene ?
4.2.3.142 (2Z,6Z)-farnesyl diphosphate exclusively uses (2Z,6Z)-farnesyl-diphosphate Solanum habrochaites PI127826 7-epizingiberene + diphosphate plus trace amounts of beta-sesquiphellandrene and curcumene ?

Subunits

EC Number Subunits Comment Organism
4.2.3.142 ? x * 84000, calculated Solanum habrochaites

Synonyms

EC Number Synonyms Comment Organism
4.2.3.142 ShZIS
-
 Solanum lycopersicum
4.2.3.142 Zis
-
 Solanum habrochaites

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.142 0.29
-
 (2Z,6Z)-farnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Solanum habrochaites

General Information

EC Number General Information Comment Organism
4.2.3.142 physiological function stable expression of short chain cis-prenyltransferase zFPS and 7-epizingiberene synthase in Solanum lycopersicum results in accumulation of 7-epizingiberene. The production of 7-epizingiberene has no repellent effect on spider mites in a choice assay, but has a strong effect on their fecundity. Mites placed on leaf disks of transgenic plants exhibit a 40% higher mortality and, after 4 d, an 81% reduction in the number of eggs Solanum habrochaites