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Literature summary extracted from

  • Augustin, M.A.; Huber, R.; Kaiser, J.T.
    Crystal structure of a DNA-dependent RNA polymerase (DNA primase) (2001), Nat. Struct. Biol., 8, 57-61.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B16
-
Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.B16 vapor diffusion in sitting drops at 20°C. Crystal structure of the catalytic primase subunit, 2.3 A resolution Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.B16 Zn2+ the zinc ion is located within the loop region between beta-strand 6 and the prominent helix alpha4. It is coordinated by Cys 106 and His 108 of the loop and Cys114and Cys117 at the beginning of helix alpha4 Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B16 Pyrococcus furiosus Q9P9H1 and Q8U4H7 Q9P9H1: small subunit, Q8U4H7: large subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.B16
-
Pyrococcus furiosus