Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reshetnikov, A.S.; Khmelenina, V.N.; Mustakhimov, I.I.; Trotsenko, Y.A.
    Genes and enzymes of ectoine biosynthesis in halotolerant methanotrophs (2011), Methods Enzymol., 495, 15-30.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.178 expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) Methylotuvimicrobium alcaliphilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.178 Cd2+
-
 Methylophaga alcalica
2.3.1.178 Cd2+
-
 Methylophaga thalassica
2.3.1.178 Cd2+
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 Cu2+
-
 Methylophaga alcalica
2.3.1.178 Cu2+
-
 Methylophaga thalassica
2.3.1.178 Zn2+
-
 Methylophaga alcalica
2.3.1.178 Zn2+
-
 Methylophaga thalassica
2.3.1.178 Zn2+
-
 Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.178 0.03
-
 acetyl-CoA pH 9.5, 25°C Methylophaga alcalica
2.3.1.178 0.037
-
 acetyl-CoA pH 9.5, 25°C Methylotuvimicrobium alcaliphilum
2.3.1.178 0.076
-
 acetyl-CoA pH 9.5, 25°C Methylophaga thalassica
2.3.1.178 0.365
-
 L-2,4-diaminobutanoate pH 9.5, 25°C Methylophaga thalassica
2.3.1.178 0.375
-
 L-2,4-diaminobutanoate pH 9.5, 25°C Methylophaga alcalica
2.3.1.178 0.465
-
 L-2,4-diaminobutanoate pH 9.5, 25°C Methylotuvimicrobium alcaliphilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.178 KCl activates, best at 0.25 M Methylotuvimicrobium alcaliphilum
2.3.1.178 additional information no activation by NaCl and KCl Methylophaga thalassica
2.3.1.178 additional information no activation by NaCl and KCl Methylophaga alcalica
2.3.1.178 NaCl activates, best at 0.1-0.2 M Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.178 20000
-
 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Methylophaga thalassica
2.3.1.178 20000
-
 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Methylotuvimicrobium alcaliphilum
2.3.1.178 20000
-
 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Methylophaga alcalica
2.3.1.178 40000
-
 recombinant His-tagged enzyme, gel filtration Methylophaga thalassica
2.3.1.178 40000
-
 recombinant His-tagged enzyme, gel filtration Methylotuvimicrobium alcaliphilum
2.3.1.178 40000
-
 recombinant His-tagged enzyme, gel filtration Methylophaga alcalica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylophaga thalassica
-
 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Halomonas elongata
-
 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylotuvimicrobium alcaliphilum
-
 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylophaga alcalica
-
 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylotuvimicrobium alcaliphilum 20Z
-
 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.178 Halomonas elongata
-
-
-
2.3.1.178 Methylophaga alcalica
-
 gene ectA
-
2.3.1.178 Methylophaga thalassica
-
 gene ectA
-
2.3.1.178 Methylotuvimicrobium alcaliphilum
-
 gene ectA
-
2.3.1.178 Methylotuvimicrobium alcaliphilum 20Z
-
 gene ectA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.178 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Methylotuvimicrobium alcaliphilum

Storage Stability

EC Number Storage Stability Organism
2.3.1.178 4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml Methylophaga thalassica
2.3.1.178 4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml Methylotuvimicrobium alcaliphilum
2.3.1.178 4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml Methylophaga alcalica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylophaga thalassica CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Halomonas elongata CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylotuvimicrobium alcaliphilum CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylophaga alcalica CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylotuvimicrobium alcaliphilum 20Z CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 additional information usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity Methylophaga thalassica ?
-
 ?
2.3.1.178 additional information usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity Halomonas elongata ?
-
 ?
2.3.1.178 additional information usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity Methylotuvimicrobium alcaliphilum ?
-
 ?
2.3.1.178 additional information usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity Methylophaga alcalica ?
-
 ?
2.3.1.178 additional information usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity Methylotuvimicrobium alcaliphilum 20Z ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.178 homodimer 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Methylophaga thalassica
2.3.1.178 homodimer 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Methylotuvimicrobium alcaliphilum
2.3.1.178 homodimer 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE Methylophaga alcalica

Synonyms

EC Number Synonyms Comment Organism
2.3.1.178 DABA acetyltransferase
-
 Methylophaga thalassica
2.3.1.178 DABA acetyltransferase
-
 Halomonas elongata
2.3.1.178 DABA acetyltransferase
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 DABA acetyltransferase
-
 Methylophaga alcalica
2.3.1.178 EctA
-
 Methylophaga thalassica
2.3.1.178 EctA
-
 Halomonas elongata
2.3.1.178 EctA
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 EctA
-
 Methylophaga alcalica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.178 20
-
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 25
-
 assay at Halomonas elongata
2.3.1.178 30 35
-
 Methylophaga thalassica
2.3.1.178 30 35
-
 Methylophaga alcalica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.178 8.2
-
-
 Halomonas elongata
2.3.1.178 8.5
-
-
 Methylophaga thalassica
2.3.1.178 9.5
-
-
 Methylotuvimicrobium alcaliphilum
2.3.1.178 9.5
-
-
 Methylophaga alcalica

General Information

EC Number General Information Comment Organism
2.3.1.178 metabolism the enzyme is involved in the pathway of ectoine biosynthesis Methylophaga thalassica
2.3.1.178 metabolism the enzyme is involved in the pathway of ectoine biosynthesis Halomonas elongata
2.3.1.178 metabolism the enzyme is involved in the pathway of ectoine biosynthesis Methylotuvimicrobium alcaliphilum
2.3.1.178 metabolism the enzyme is involved in the pathway of ectoine biosynthesis Methylophaga alcalica