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Literature summary extracted from

  • Chen, H.; Yuan, Y.
    Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase (2010), J. Mol. Cell Biol., 2, 366-374.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.257 gene Mj1640, expression of wild-type and selenmethionine-labeled enzyme as N-terminally His-tagged protein in Escherichia coli strain Bl21(DE3) Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.257 purified recombinant wild-type and selenmethionine-labeled DUF358/Mj1640 in complex with S-adenosyl-L-methionine, at 20°C, mixing of 0.001 ml of protein solution with 0.001 l of reservoir containing 20% PEG 3350, 25 mM NH4F, 20 mM MgCl2, 100 mM Tris, pH 7.5, X-ray diffraction structure determination and analysis at 1.4 A resolution Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.257 Methanocaldococcus jannaschii Q59034 gene Mj1640
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.257 recombinant His-tagged wild-type and selenmethionine-labeled Mj1640 from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography and gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.257 additional information SAM-binding pocket Asp157 or Glu183 from its own monomer or Ser43 from the associate monomer probably plays the catalytic role for RNA methylation, RNA-binding residues and putative active site, overview Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.257 dimer Mj1640 forms a dimer, which is mediated by two parallel pairs of alpha-helices oriented almost perpendicular to each other Methanocaldococcus jannaschii
2.1.1.257 More the enzyme shows a single domain structure consisting of eight-stranded beta-sheets sandwiched by six alpha-helices at both sides. Mj1640 has limited structural extension at its N-terminus, which is unique to this family member, structure modeling based on the crystal structure, overview Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.1.1.257 DUF358/Mj1640
-
 Methanocaldococcus jannaschii
2.1.1.257 m1-pseudouridine methyltransferase
-
 Methanocaldococcus jannaschii
2.1.1.257 m1G
-
 Methanocaldococcus jannaschii
2.1.1.257 M3U
-
 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.1.1.257 evolution the enzyme is a SPOUT-class RNA methyltransferase and belongs to the DUF358 family. The DUF358/Mj1640 protein shows close structural relationship to Nep1, a pseudouridine-N1-specific rRNA methyltransferase Methanocaldococcus jannaschii