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Literature summary extracted from

  • Rosini, E.; Monelli, C.; Pollegioni, L.; Riva, S.; Monti, D.
    On the substrate preference of glutaryl acylases (2012), J. Mol. Catal. B, 76, 52-58.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.93 H296S/H309S the mutant also shows activity with cephalosporin C (3fold increase compared to the wild type enzyme) Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.93 glutaryl-7-aminocephalosporanic acid substrate inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.93 0.7
-
 adipyl-7-aminodesacetoxycephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 1
-
 glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 1.3
-
 glutaryl-7-aminodesacetoxycephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 1.5
-
 glutaryl-7-aminodesacetoxycephalosporanic acid recombinant enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 1.5
-
 glutaryl-7-aminocephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 2.2
-
 glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid recombinant enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 2.5
-
 glutaryl-7-aminocephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 2.8
-
 succinyl-7-aminocephalosporanic acid recombinant enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 2.8
-
 succinyl-7-aminocephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 3
-
 glutaryl-7-aminocephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 3.3
-
 glutaryl-7-aminocephalosporanic acid recombinant enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 3.8
-
 adipyl-7-aminodesacetoxycephalosporanic acid recombinant enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 4.3
-
 adipyl-7-aminocephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 4.4
-
 adipyl-7-aminocephalosporanic acid recombinant enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 5.4
-
 succinyl-7-aminocephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 5.4
-
 glutaryl-7-aminocephalosporanic acid recombinant enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 6.9
-
 glutaryl-7-aminocephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 7.5
-
 adipyl-7-aminocephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 7.9
-
 adipyl-7-aminodesacetoxycephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 8.7
-
 glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 9.5
-
 cephalosporin C wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 12.2
-
 cephalosporin C mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 14.1
-
 glutaryl-7-aminodesacetoxycephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 16.1
-
 pentanoyl-7-aminocephalosporanic acid mutant enzyme H296S/H309S, at pH 8.0 and 25°C Pseudomonas sp.
3.5.1.93 17.4
-
 pentanoyl-7-aminocephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 adipyl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. adipate + 7-aminocephalosporanate
-
 ?
3.5.1.93 adipyl-7-aminodesacetoxycephalosporanic acid + H2O 57.4% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. adipate + 7-aminodesacetoxycephalosporanate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Pseudomonas sp. cephalosporamic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid + H2O 81.9% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + 3-chloro-7-aminodesacetoxycephalosporanate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O 100% activity Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O 9.9% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + 7-aminocephalosporanate
-
 ?
3.5.1.93 glutaryl-7-aminodesacetoxycephalosporanic acid + H2O 71.5% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + 7-aminodesacetoxycephalosporanate
-
 ?
3.5.1.93 glutaryl-D-Phe-Gly + H2O about 25% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + D-Phe-Gly
-
 ?
3.5.1.93 glutaryl-glycine + H2O less than 5% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + glycine
-
 ?
3.5.1.93 glutaryl-L-Ala + H2O about 25% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + L-Ala
-
 ?
3.5.1.93 glutaryl-L-Phe + H2O about 10% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + L-Phe
-
 ?
3.5.1.93 glutaryl-L-Phe-Gly + H2O about 50% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. glutarate + L-Phe-Gly
-
 ?
3.5.1.93 additional information the recombinant enzyme shows no activity towards cephalosporin C, butanoyl-7-aminodesacetoxycephalosporanic acid and pentanoyl-7-aminodesacetoxycephalosporanic acid Pseudomonas sp. ?
-
 ?
3.5.1.93 pentanoyl-7-aminocephalosporanic acid + H2O about 2.4% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. pentanoate + 7-aminocephalosporanate
-
 ?
3.5.1.93 succinyl-7-aminocephalosporanic acid + H2O 25.8% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid Pseudomonas sp. succinate + 7-aminocephalosporanate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 GAR commercial preparation Pseudomonas sp.
3.5.1.93 glutaryl acylase
-
 Pseudomonas sp.
3.5.1.93 glutaryl-7-ACA acylase
-
 Pseudomonas sp.
3.5.1.93 VAC
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.93 21
-
 glutaryl-7-aminocephalosporanic acid wild type enzyme, at pH 8.0 and 25°C Pseudomonas sp.