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Literature summary extracted from

  • Niehaus, T.D.; Kinison, S.; Okada, S.; Yeo, Y.S.; Bell, S.A.; Cui, P.; Devarenne, T.P.; Chappell, J.
    Functional identification of triterpene methyltransferases from Botryococcus braunii race B (2012), J. Biol. Chem., 287, 8163-8173.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.262 overexpression of TMT-1 and TMT-2 in Saccharomyces cerevisiae microsomes leading to mono- and dimethylated products, co-expression of various TMT and SMT-like genes in Saccharomyces cerevisiae strain TN-7 Botryococcus braunii
2.1.1.263 overexpression of TMT-3 in Saccharomyces cerevisiae microsomes leading to mono- and dimethylated products, co-expression of various TMT and SMT-like genes in Saccharomyces cerevisiae strain TN-7 Botryococcus braunii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.262 additional information
-
 additional information Michaelis-Menten enzyme kinetics for TMT-1 and TMT-2 Botryococcus braunii
2.1.1.263 additional information
-
 additional information Michaelis-Menten enzyme kinetics for TMT-3 Botryococcus braunii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.262 membrane
-
 Botryococcus braunii 16020
-
2.1.1.263 membrane
-
 Botryococcus braunii 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.262 2 S-adenosyl-L-methionine + squalene Botryococcus braunii overall reaction 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene
-
 ir
2.1.1.262 2 S-adenosyl-L-methionine + squalene Botryococcus braunii race B overall reaction 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene
-
 ir
2.1.1.262 S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene
-
 ?
2.1.1.262 S-adenosyl-L-methionine + squalene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene
-
 ?
2.1.1.263 2 S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii overall reaction 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ir
2.1.1.263 S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
2.1.1.263 S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
2.1.1.263 S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
-
 ?
2.1.1.263 S-adenosyl-L-methionine + C30 botryococcene Botryococcus braunii
-
 S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.96 Botryococcus braunii
-
 race B
-
1.3.1.97 Botryococcus braunii
-
 race B
-
2.1.1.262 Botryococcus braunii
-
-
-
2.1.1.262 Botryococcus braunii race B
-
-
-
2.1.1.263 Botryococcus braunii
-
-
-
2.1.1.263 Botryococcus braunii race B
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.262 8.56
-
 recombinant TMT-1, pH 7.5, 37°C Botryococcus braunii
2.1.1.262 143.7
-
 recombinant TMT-2, pH 7.5, 37°C Botryococcus braunii
2.1.1.263 7.25
-
 recombinant enzyme, pH 7.5, 37°C Botryococcus braunii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.96 additional information SSL-2 cannot efficiently use farnesyl diphosphate as a substrate Botryococcus braunii ?
-
 ?
1.3.1.96 additional information SSL-2 cannot efficiently use farnesyl diphosphate as a substrate Botryococcus braunii race B ?
-
 ?
1.3.1.96 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii squalene + diphosphate + NADP+
-
 ir
1.3.1.96 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii race B squalene + diphosphate + NADP+
-
 ir
1.3.1.97 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii C30 botryococcene + NADP+ + diphosphate
-
 ?
1.3.1.97 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii race B C30 botryococcene + NADP+ + diphosphate
-
 ?
2.1.1.262 2 S-adenosyl-L-methionine + squalene overall reaction Botryococcus braunii 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene
-
 ir
2.1.1.262 2 S-adenosyl-L-methionine + squalene overall reaction Botryococcus braunii race B 2 S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene
-
 ir
2.1.1.262 additional information mono- and dimethylated products with terminal carbon C-3 as the atomic acceptor sites for the methyl additions to squalene, NMR analysis, overview TMT-1 and TMT-2 show only low activity with C30 botryococcene Botryococcus braunii ?
-
 ?
2.1.1.262 additional information mono- and dimethylated products with terminal carbon C-3 as the atomic acceptor sites for the methyl additions to squalene, NMR analysis, overview TMT-1 and TMT-2 show only low activity with C30 botryococcene Botryococcus braunii race B ?
-
 ?
2.1.1.262 S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3,22-dimethyl-1,2,23,24-tetradehydro-2,3,22,23-tetrahydrosqualene
-
 ?
2.1.1.262 S-adenosyl-L-methionine + squalene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrosqualene
-
 ?
2.1.1.263 2 S-adenosyl-L-methionine + C30 botryococcene overall reaction Botryococcus braunii 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ir
2.1.1.263 additional information mono- and dimethylated products with terminal carbons C-22/C-20 as the atomic acceptor sites for the methyl additions to botryococcene, NMR analysis, overview. TMT-3 shows only low activity with squalene Botryococcus braunii ?
-
 ?
2.1.1.263 S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
2.1.1.263 S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
-
 ?
2.1.1.263 S-adenosyl-L-methionine + C30 botryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
-
 ?
2.1.1.263 S-adenosyl-L-methionine + C30 botryococcene
-
 Botryococcus braunii S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.96 squalene synthase-like 2
-
 Botryococcus braunii
1.3.1.96 SSL-2
-
 Botryococcus braunii
1.3.1.97 SSL-3
-
 Botryococcus braunii
2.1.1.262 triterpene methyltransferase
-
 Botryococcus braunii
2.1.1.263 TMT-3
-
 Botryococcus braunii
2.1.1.263 triterpene methyltransferase-3
-
 Botryococcus braunii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.262 37
-
 assay at Botryococcus braunii
2.1.1.263 37
-
 assay at Botryococcus braunii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.262 7.5
-
 assay at Botryococcus braunii
2.1.1.263 7.5
-
 assay at Botryococcus braunii

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.262 S-adenosyl-L-methionine
-
 Botryococcus braunii
2.1.1.263 S-adenosyl-L-methionine
-
 Botryococcus braunii

General Information

EC Number General Information Comment Organism
2.1.1.262 metabolism TMT-1 and TMT-2 exhibit preference for squalene as the methyl acceptor substrate Botryococcus braunii
2.1.1.263 metabolism TMT-3 shows a striking preference for botryococcene as its methyl acceptor substrate Botryococcus braunii