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Literature summary extracted from
- Isolation of Rhizobacteria from Jatropha curcas and characterization of produced ACC deaminase (2012), J. Basic Microbiol., 52, 285-295.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.99.7 | gene acdS, DNA and amino acid sequence determination, phylogenetic analysis using 16S RNA genetic sequence | Enterobacter cloacae |
| 3.5.99.7 | gene acdS, DNA and amino acid sequence determination, phylogenetic analysis using 16S RNA genetic sequence | Enterobacter cancerogenus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.99.7 | 2.08 | - |
1-aminocyclopropane-1-carboxylate | pH 7.5, 30°C | Enterobacter cloacae |  |
| 3.5.99.7 | 12.5 | - |
1-aminocyclopropane-1-carboxylate | pH 7.5, 30°C | Enterobacter cancerogenus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.7 | K2HPO4 | results in highest activity | Enterobacter cloacae | |
| 3.5.99.7 | MgSO4 | results in highest activity | Enterobacter cancerogenus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cloacae | - |
2-oxobutanoate + NH3 | - |
? | |
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cancerogenus | - |
2-oxobutanoate + NH3 | - |
? | |
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cancerogenus MSA2 | - |
2-oxobutanoate + NH3 | - |
? | |
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | Enterobacter cloacae MSA1 | - |
2-oxobutanoate + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.99.7 | Enterobacter cancerogenus | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
| 3.5.99.7 | Enterobacter cancerogenus MSA2 | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
| 3.5.99.7 | Enterobacter cloacae | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
| 3.5.99.7 | Enterobacter cloacae MSA1 | - |
recovered from the rhizosphere of Jatropha sp., gene acdS | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.99.7 | native enzyme by ammonium sulfate fractionation, and anion exchange chromatography | Enterobacter cloacae |
| 3.5.99.7 | native enzyme by ammonium sulfate fractionation, and anion exchange chromatography | Enterobacter cancerogenus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cloacae | 2-oxobutanoate + NH3 | - |
? | |
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cancerogenus | 2-oxobutanoate + NH3 | - |
? | |
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cancerogenus MSA2 | 2-oxobutanoate + NH3 | - |
? | |
| 3.5.99.7 | 1-aminocyclopropane-1-carboxylate + H2O | - |
Enterobacter cloacae MSA1 | 2-oxobutanoate + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.99.7 | multimer | x * 35000-42000, SDS-PAGE | Enterobacter cloacae |
| 3.5.99.7 | multimer | x * 35000-42000, SDS-PAGE | Enterobacter cancerogenus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.99.7 | ACC deaminase | - |
Enterobacter cloacae |
| 3.5.99.7 | ACC deaminase | - |
Enterobacter cancerogenus |
| 3.5.99.7 | ACC-deaminase | - |
Enterobacter cloacae |
| 3.5.99.7 | ACC-deaminase | - |
Enterobacter cancerogenus |
| 3.5.99.7 | More | formerly EC 4.1.99.4 | Enterobacter cloacae |
| 3.5.99.7 | More | formerly EC 4.1.99.4 | Enterobacter cancerogenus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.7 | 28 | - |
- |
Enterobacter cloacae |
| 3.5.99.7 | 28 | - |
- |
Enterobacter cancerogenus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.7 | 25 | 50 | activity range, optimum activity at 28°C, rapid decrease in activity above 30°C, no activity at 60°C | Enterobacter cloacae |
| 3.5.99.7 | 25 | 50 | activity range, optimum activity at 28°C, rapid decrease in activity above 30°C, no activity at 60°C | Enterobacter cancerogenus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.7 | 7.5 | - |
- |
Enterobacter cloacae |
| 3.5.99.7 | 7.5 | - |
- |
Enterobacter cancerogenus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.7 | pyridoxal 5'-phosphate | tightly bound to the enzyme in the amount of approximately one molecule per subunit | Enterobacter cloacae | |
| 3.5.99.7 | pyridoxal 5'-phosphate | tightly bound to the enzyme in the amount of approximately one molecule per subunit | Enterobacter cancerogenus | |
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