EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.5.1 | DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain BL21-Gold(DE3)/pOK101/pTf16, no cleavage of the C-terminal peptide of the enzyme in the recombinant bacteria | Aspergillus niger |
EC Number | General Stability | Organism |
---|---|---|
3.5.5.1 | the purified native and recombinant enzyme are stabilized by glycine at 1% w/v, sucrose at 10% w/v, D-glucose at 10% w/v, trehalose at 10% w/v, D-sorbitol at 10% w/v, xylitol at 10% w/v, D-myo-inositol at 10%, D-glycerol at 10% w/v, and bovine serum albumin at 0.1-1.0% w/v, in different extents, overview | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.1 | 38500 | - |
x * 42700, recombinant enzyme, SDS-PAGE, x * 38500, native enzyme, SDS-PAGE, the multimeric nitrilase is composed of 12-16 subunits, mass spectrometry, analytical centrifugation, and dynamic light scattering, modelling | Aspergillus niger |
3.5.5.1 | 42700 | - |
x * 42700, recombinant enzyme, SDS-PAGE, x * 38500, native enzyme, SDS-PAGE, the multimeric nitrilase is composed of 12-16 subunits, mass spectrometry, analytical centrifugation, and dynamic light scattering, modelling | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.1 | Aspergillus niger | A9QXE0 | - |
- |
3.5.5.1 | Aspergillus niger K10 | A9QXE0 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.5.5.1 | proteolytic modification | the mature enzyme is reduced in size by about 4 kDa compared to the unprocessed enzyme, cleavage of the C-terminal peptide | Aspergillus niger |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.1 | recombinant enzyme 2fold from Escherichia coli strain BL21-Gold(DE3)/pOK101/pTf16, further purification of the refolded recombinant enzyme after 1 month storage by gel filtration | Aspergillus niger |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.5.5.1 | recombinant enzyme is fully denatured in 6 M guanidine-HCl and 2 M Tris-carboxyethylphosphine and refolded in vitro | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.1 | 2-cyanopyridine + 2 H2O | - |
Aspergillus niger | pyridine 2-carboxylic acid + NH3 | - |
? | |
3.5.5.1 | 2-cyanopyridine + 2 H2O | - |
Aspergillus niger K10 | pyridine 2-carboxylic acid + NH3 | - |
? | |
3.5.5.1 | 3-chlorobenzonitrile + 2 H2O | - |
Aspergillus niger | 3-chlorobenzoic acid + NH3 | - |
? | |
3.5.5.1 | 3-cyanopyridine + 2 H2O | - |
Aspergillus niger | nicotinic acid + NH3 | - |
? | |
3.5.5.1 | 3-cyanopyridine + 2 H2O | - |
Aspergillus niger K10 | nicotinic acid + NH3 | - |
? | |
3.5.5.1 | 4-chlorobenzonitrile + 2 H2O | - |
Aspergillus niger | 4-chlorobenzoic acid + NH3 | - |
? | |
3.5.5.1 | 4-cyanopyridine + 2 H2O | - |
Aspergillus niger | pyridine 4-carboxylic acid + NH3 | - |
? | |
3.5.5.1 | 4-cyanopyridine + 2 H2O | - |
Aspergillus niger K10 | pyridine 4-carboxylic acid + NH3 | - |
? | |
3.5.5.1 | additional information | substrate specificity and chemoselectivity of native and recombinant enzymes, no activity of both with 2-chlorobenzonitrile, 2-phenylpropionitrile, overview | Aspergillus niger | ? | - |
? | |
3.5.5.1 | additional information | substrate specificity and chemoselectivity of native and recombinant enzymes, no activity of both with 2-chlorobenzonitrile, 2-phenylpropionitrile, overview | Aspergillus niger K10 | ? | - |
? | |
3.5.5.1 | phenylacetonitrile + 2 H2O | low activity with the native enzyme, poor activity with the recombinant enzyme | Aspergillus niger | phenylacetic acid + NH3 | - |
? | |
3.5.5.1 | phenylacetonitrile + 2 H2O | low activity with the native enzyme, poor activity with the recombinant enzyme | Aspergillus niger K10 | phenylacetic acid + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.5.1 | More | homology modelling and molecular dynamics, overview | Aspergillus niger |
3.5.5.1 | multimer | x * 42700, recombinant enzyme, SDS-PAGE, x * 38500, native enzyme, SDS-PAGE, the multimeric nitrilase is composed of 12-16 subunits, mass spectrometry, analytical centrifugation, and dynamic light scattering, modelling | Aspergillus niger |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.5.1 | additional information | native and recombinant Escherichia coli-expressed enzymes differ in substrate specificity, acid/amide ratio, reaction optima and stability | Aspergillus niger |