Literature summary extracted from
Griffin, D.H.; Richmond, T.K.; Sanchez, C.; Moller, A.J.; Breece, R.M.; Tierney, D.L.; Bennett, B.; Crowder, M.W.
Structural and kinetic studies on metallo-beta-lactamase IMP-1 (2011), Biochemistry, 50, 9125-9134.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.2.6 |
expression in Escherichia coli |
Serratia marcescens |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.5.2.6 |
0.028 |
- |
nitrocefin |
pH 7.5, 25°C |
Serratia marcescens |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.5.2.6 |
Co2+ |
UV-vis studies on IMP-1 containing 1 equivalent of Co(II) show a strong ligand-to-metal charge transition at 340 nm, and the intensity of this feature increases when the second equivalent of Co(II) is added to the enzyme |
Serratia marcescens |
|
3.5.2.6 |
additional information |
metal-binding to metal-free IMP-1 follows a positive-cooperative mode |
Serratia marcescens |
|
3.5.2.6 |
Zn2+ |
enzyme binds 2 equivalents of Zn2+ |
Serratia marcescens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.2.6 |
Serratia marcescens |
P52699 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.2.6 |
nitrocefin + H2O |
IMP-1 does not stabilize a nitrocefin-derived reaction intermediate, rather, the enzyme follows a simple Michaelis mechanism to hydrolyze nitrocefin |
Serratia marcescens |
(2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.5.2.6 |
IMP-1 |
- |
Serratia marcescens |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.5.2.6 |
135 |
- |
nitrocefin |
pH 7.5, 25°C |
Serratia marcescens |
|