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Literature summary extracted from

  • Ganapathy, K.; Kanagasabai, R.; Nguyen, T.T.; Nes, W.D.
    Purification, characterization and inhibition of sterol C24-methyltransferase from Candida albicans (2011), Arch. Biochem. Biophys., 505, 194-201.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.41 24(R,S),25-epiminolanosterol
-
 Candida albicans
2.1.1.41 24-thialanosterol
-
 Candida albicans
2.1.1.41 25-azalanosterol
-
 Candida albicans
2.1.1.41 26,27-dehydrozymosterol
-
 Candida albicans
2.1.1.41 cholesta-8,24(28)-dienol
-
 Candida albicans
2.1.1.41 ergosta-5,7,22-trienol
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.41 0.05
-
 Zymosterol pH 7.5, 30°C Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.41 43000
-
 SDS-PAGE Candida albicans
2.1.1.41 178000
-
 gel filtration Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.41 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.41 using anion exchange chromatography, gel filtration and fast performance liquid chromatography using a Mono Q column Candida albicans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.41 S-adenosyl-L-methionine + zymosterol
-
 Candida albicans S-adenosyl-L-homocysteine + fecosterol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.41 tetramer 4 * 43000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
2.1.1.41 24-SMT
-
 Candida albicans
2.1.1.41 sterol C24-methyltransferase
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.41 30
-
 assay at Candida albicans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.41 0.01
-
 Zymosterol pH 7.5, 30°C Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.41 7.5
-
 assay at Candida albicans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.41 0.000009
-
 26,27-dehydrozymosterol pH 7.5, 30°C Candida albicans
2.1.1.41 0.000011
-
 24(R,S),25-epiminolanosterol pH 7.5, 30°C Candida albicans
2.1.1.41 0.000054
-
 25-azalanosterol pH 7.5, 30°C Candida albicans
2.1.1.41 0.02
-
 ergosta-5,7,22-trienol pH 7.5, 30°C Candida albicans
2.1.1.41 0.072
-
 cholesta-8,24(28)-dienol pH 7.5, 30°C Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.41 0.003
-
 pH 7.5, 30°C Candida albicans 25-azalanosterol
2.1.1.41 0.005
-
 pH 7.5, 30°C Candida albicans 24(R,S),25-epiminolanosterol
2.1.1.41 0.02
-
 pH 7.5, 30°C Candida albicans 24-thialanosterol