Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rampioni, G.; Bertani, I.; Pillai, C.R.; Venturi, V.; Zennaro, E.; Leoni, L.
    Functional characterization of the quorum sensing regulator RsaL in the plant-beneficial strain Pseudomonas putida WCS358 (2012), Appl. Environ. Microbiol., 78, 726-734.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.184 gene lasI, genetic organization and las genetic locus Pseudomonas aeruginosa
2.3.1.184 gene ppuI, genetic organization and ppu genetic locus Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.184 9-hydroxy-2-propyl-4H-pyrido[1,2-a]pyrimidin-4-one
-
 Pseudomonas putida
2.3.1.184 N-(3-oxocyclohex-1-en-1-yl)octanamide
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.184 3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine Pseudomonas aeruginosa
-
 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
2.3.1.184 3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine Pseudomonas putida
-
 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
2.3.1.184 3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine Pseudomonas putida WCS358
-
 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.184 Pseudomonas aeruginosa
-
 gene lasI
-
2.3.1.184 Pseudomonas putida
-
 gene ppuI
-
2.3.1.184 Pseudomonas putida WCS358
-
 gene ppuI
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.184 3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
-
 Pseudomonas aeruginosa [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
2.3.1.184 3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
-
 Pseudomonas putida [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?
2.3.1.184 3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
-
 Pseudomonas putida WCS358 [acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.184 LasI
-
 Pseudomonas aeruginosa
2.3.1.184 LasI synthase
-
 Pseudomonas aeruginosa
2.3.1.184 PppuI
-
 Pseudomonas putida

Expression

EC Number Organism Comment Expression
2.3.1.184 Pseudomonas putida RsaLWCS displays a strong repressive effect on the promoter of the quorum sensing signal synthase gene, ppuI. Purified RsaLWCS specifically binds to ppuI on a DNA region overlapping the predicted sigma70-binding site, but the RsaLWCS protein is not able to form stable complexes efficiently with a probe encompassing the ppuI promoter, the RsaLWCS/PppuI complex is not stable in vitro, RsaLWCS needs a molecular interactor/cofactor specific for Pseudomonas putida WCS358 to repress ppuI transcription. RsaLWCS regulates siderophore-mediated growth limitation of plant pathogens and biofilmcformation, two processes relevant for plant growth-promoting activity. Mutational analysis using a chimeric RsaLWCS mutant, RsaLWCS molecular mechanism, overview down
2.3.1.184 Pseudomonas aeruginosa RsaLWCS does not show a repressive effect on the promoter of the QS signal synthase gene lasI in Pseudomonas aeruginosa. RsaLWCS formed stable complexes with the promoter of lasI, the gene orthologous to ppuI. RsaLWCS regulates siderophore-mediated growth limitation of plant pathogens and biofilm formation, two processes relevant for plant growth-promoting activity. RsaL binds close to LasR on the same bidirectional promoter, repressing the transcription of both the lasI and rsaL genes. Molecular mechanism, overview down

General Information

EC Number General Information Comment Organism
2.3.1.184 physiological function quorum sensing systems rely on a signal receptor and a synthase producing N-acyl-homoserine lactone(s) as the signal molecule(s). The rsaL gene, located between the signal receptor and synthase genes, encodes a repressor limiting signal synthase expression and hence signal molecule production, molecular mechanism of action of the RsaL protein in the plant growth-promoting rhizobacterium Pseudomonas putida WCS358, overview Pseudomonas putida
2.3.1.184 physiological function quorum sensing systems rely on a signal receptor and a synthase producing N-acyl-homoserine lactone(s) as the signal molecule(s). The rsaL gene, located between the signal receptor and synthase genes, encodes a repressor limiting signal synthase expression and hence signal molecule production, molecular mechannism, overview Pseudomonas aeruginosa