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Literature summary extracted from

  • Sevrioukova, I.F.; Poulos, T.L.
    Putidaredoxin reductase, a new function for an old protein (2002), J. Biol. Chem., 277, 25831-25839.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.5
-
Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.5 additional information expression of wild-type as His-tagged protein. Molecular, spectral, and electron transferring properties of recombinant His6-Pdr to artificial and native electron acceptors are similar to those of the wild-type protein. Contrary to wild-type, under anaerobic conditions, NAD+ induces in His6 Pdr spectral changes indicative of flavin reduction and formation of the charge transfer complex between the reduced FAD and NAD+ Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.5 Pseudomonas putida P16640
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.5 Fe(CN)62- + NAD+
-
Pseudomonas putida Fe(CN)63- + NADH + H+
-
?
1.18.1.5 additional information wild-type and His6 Pdr are able to function as NAD(H)-dependent dithiol/disulfide oxidoreductases catalyzing both forward and reverse reactions, NAD+-dependent oxidation of thiols, and NADH-dependent reduction of disulfides. This function of the flavoprotein can be dissociated from electron transfer to putidaredoxin Pseudomonas putida ?
-
?
1.18.1.5 reduced 2,6-dichlorophenolindophenol + NAD+
-
Pseudomonas putida oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
?
1.18.1.5 reduced putidaredoxin + NAD+
-
Pseudomonas putida oxidized putidaredoxin + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.18.1.5 dimer both WT and His6-Pdr undergo a monomer-dimer association-dissociation Pseudomonas putida
1.18.1.5 monomer both WT and His6-Pdr undergo a monomer-dimer association-dissociation Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.18.1.5 Pdr
-
Pseudomonas putida
1.18.1.5 putidaredoxin reductase
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.5 126
-
Fe(CN)62- pH 8.0, 25°C Pseudomonas putida
1.18.1.5 264
-
reduced putidaredoxin pH 8.0, 25°C Pseudomonas putida
1.18.1.5 671
-
Fe(CN)62- pH 8.0, 25°C Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.5 NAD+ both WT and His6-Pdr are partially present as an NAD+-bound form Pseudomonas putida