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Literature summary extracted from

  • Tallant, T.; Paul, L.; Krzycki, J.
    The MtsA subunit of the methylthiol:coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer (2001), J. Biol. Chem., 276, 4485-4493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.251 gene mtsA, functional expression in Escherichia coli strain BL21(DE3)pLysS, MtsA is expressed largely in an insoluble form Methanosarcina barkeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.251 Dimethylsulfide inhibits methylcobalamin:coenzyme methyl transfer by MtsA. Inhibition by dimethylsulfide is mixed with respect to methylcobalamin, but competitive with coenzyme M Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.251 additional information
-
 additional information kinetic analysis, overview Methanosarcina barkeri
2.1.1.251 5.5
-
 a [Co(I) methylated-thiol-specific corrinoid protein] recombinant enzyme, pH 7.0, 37°C Methanosarcina barkeri
2.1.1.251 10.8
-
 coenzyme M recombinant enzyme, pH 7.0, 37°C Methanosarcina barkeri
2.1.1.251 33
-
 Dimethylsulfide recombinant enzyme, pH 7.0, 37°C Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.251 Zn2+ one zinc atom per polypeptide Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.251 41000
-
 2 * 41000, recombinant enzyme, SDS-PAGE Methanosarcina barkeri
2.1.1.251 77000
-
 recombinant enzyme, gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.251 dimethylsulfide + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + methanethiol
-
 ?
2.1.1.251 methanethiol + coenzyme M Methanosarcina barkeri
-
 methyl-CoM + hydrogen sulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.251 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.251 recombinant MtsA solubilized from inclusion bodies after expression in Escherichia coli strain BL21(DE3) Methanosarcina barkeri

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.1.1.251 recombinant enzyme from inclusion bodies harvested from cells solubilized using 6 M guanidine hydrochloride, 50 mM DTT, and 20 mM 2-mercaptoethanol. The solubilized protein is diluted 60fold with protein refolding at room temperature for 24 h in refolding buffer consisting of 500 mM Tris, pH 8.0, 1 M KCl, 10 mM DTT, 10 mM 2-mercaptoethanol, and 100 mM ZnCl2. Refolding buffers containing 500 mM guanidine hydrochloride, 33 mM CHAPS, 0.5% Triton X-100, 5 mM SDS, or 20% glycerol do not produce active methyltransferase, nor do refolding at 4°C in the optimal refolding buffer Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.251 0.078
-
 purified recombinant enzyme, substrate is dimethylsulfide, pH 7.0, 37°C Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.251 a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein]
-
 ?
2.1.1.251 dimethylsulfide + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + methanethiol
-
 ?
2.1.1.251 methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide
-
 ?
2.1.1.251 methanethiol + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + hydrogen sulfide
-
 ?
2.1.1.251 additional information MtsA acts both to demethylate dimethylsulfide and to methylate CoM using non-protein-bound cobalamin as an intermediate methyl carrier, dependence of the rate of the MtsA-catalyzed methylcobalamin:CoM methyl transfer reaction on methylcobalamin, overview. The subunit carries out both CoM methylation and DMS demethylation and can account for both corrinoid-dependent methyltransferase activities of the methylthiol:CoM methyltransferase. A model for the native methylthiol:coenzyme M methyltransferase in which MtsA mediates the methylation of corrinoid bound to MtsB with dimethylsulfide and subsequently demethylates MtsB-bound corrinoid with coenzyme M, overview Methanosarcina barkeri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.251 homodimer 2 * 41000, recombinant enzyme, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.251 methylthiol:coenzyme M methyltransferase
-
 Methanosarcina barkeri
2.1.1.251 MtsA
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.251 22
-
 Cob(I)alamin methylation assay at Methanosarcina barkeri
2.1.1.251 37
-
 methylcobalamin:CoM methyltransferase activity assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.251 7
-
 assay at Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.251 additional information no corrinoid cofactor bound to the enzyme Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.251 physiological function methanogenesis from dimethylsulfide requires the intermediate methylation of coenzyme M. This reaction is catalyzed by a methylthiol:coenzymeMmethyltransferase composed of two polypeptides, MtsA, which is a methylcobalamin:coenzyme M methyltransferase, and MtsB, which is homologous to a class of corrinoid proteins involved in methanogenesis. MtsA is an active methylcobalamin:coenzyme M methyltransferase, but also methylates cob(I)alamin with dimethylsulfide, yielding equimolar methylcobalamin and methanethiol Methanosarcina barkeri