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Literature summary extracted from

  • Sosedov, O.; Matzer, K.; Buerger, S.; Kiziak, C.; Baum, S.; Altenbuchner, J.; Chmura, A.; van Rantwijk, F.; Stolz, A.
    Synthesis of enantiomerically pure (S)-mandelic acid using anoxynitrilasenitrilase bienzymatic cascade: a nitrilase surprisingly shows nitrile hydratase activity (2009), Adv. Synth. Catal., 351, 1531 - 1538.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.5 expression in Escherichia coli JM 109, simultanuously with cassava (Manihot esculenta) oxynitrilase Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Pseudomonas fluorescens Q5EG61
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3.5.5.5 Pseudomonas fluorescens EBC191 Q5EG61
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 (S)-mandelonitrile + 2 H2O
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 Pseudomonas fluorescens (S)-mandelic acid + NH3 the product is 94% enantiomeric pure (S)-isomer, the formation of ca 50% (S)-mandeloamide is observed ?
3.5.5.5 (S)-mandelonitrile + 2 H2O
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 Pseudomonas fluorescens EBC191 (S)-mandelic acid + NH3 the product is 94% enantiomeric pure (S)-isomer, the formation of ca 50% (S)-mandeloamide is observed ?

General Information

EC Number General Information Comment Organism
3.5.5.5 metabolism involved in the biosynthesis of adenosylcobalamin Pseudomonas fluorescens