Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Colussi, F.; Textor, L.C.; Serpa, V.; Maeda, R.N.; Pereira, N.; Polikarpov, I.
    Purification, crystallization and preliminary crystallographic analysis of the catalytic domain of the extracellular cellulase CBHI from Trichoderma harzianum (2010), Acta Crystallogr. Sect. F, 66, 1041-1044.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 purified catalytic core domain, sitting drop vapour diffusion technique, 0.001 ml of 8 mg/ml protein in 50 mM Tris-HCl, pH 7.0, are mixed with 0.001 ml of precipitation solution containing 35% PEG 4000, X-ray diffraction structure determination and analysis at 2.9-3.0 A resolution Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 46000
-
 1 * 46000, SDS-PAGE Trichoderma harzianum
3.2.1.4 50000
-
 gel filtration Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Trichoderma harzianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 native enzyme from fermentation medium by anion exchange chromatography, ultrafiltration and hydrophobic interaction chromatography followed by cleavage through papain to isolate the catalytic core domain, and gel filtration Trichoderma harzianum

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 46000, SDS-PAGE Trichoderma harzianum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CBHI
-
 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
 assay at Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
 assay at Trichoderma harzianum