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Literature summary extracted from

  • Fawzi, E.M.
    Highly thermostable xylanase purified from Rhizomucor miehei NRL 3169 (2011), Acta Biol. Hung., 62, 85-94.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 industry the enzyme is a potentially strong candidate for industrial and commercial application in pulp bleaching Rhizomucor miehei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ba2+
-
 Rhizomucor miehei
3.2.1.8 DTT
-
 Rhizomucor miehei
3.2.1.8 EDTA
-
 Rhizomucor miehei
3.2.1.8 Fe3+
-
 Rhizomucor miehei
3.2.1.8 Hg2+
-
 Rhizomucor miehei
3.2.1.8 Mg2+
-
 Rhizomucor miehei
3.2.1.8 Mn2+
-
 Rhizomucor miehei
3.2.1.8 Zn2+
-
 Rhizomucor miehei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular the enzyme is secreted Rhizomucor miehei
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ca2+ activates Rhizomucor miehei
3.2.1.8 Cu2+ activates Rhizomucor miehei
3.2.1.8 K+ activates Rhizomucor miehei
3.2.1.8 Na+ activates Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 27000
-
 x * 27000, SDS-PAGE Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Rhizomucor miehei
-
-
-
3.2.1.8 Rhizomucor miehei NRL 3169
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 native enzyme 29.1fold to homogeneity by ammonium sulfate fractionation, gel filtration and anion exchange chromatography Rhizomucor miehei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 1849
-
 purified enzyme, substrate birch wood xylan, pH 5.0, 75°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birch wood xylan + H2O
-
 Rhizomucor miehei ?
-
 ?
3.2.1.8 birch wood xylan + H2O
-
 Rhizomucor miehei NRL 3169 ?
-
 ?
3.2.1.8 additional information no activity with cellulose, cellobiose, and carboxymethyl cellulose Rhizomucor miehei ?
-
 ?
3.2.1.8 additional information no activity with cellulose, cellobiose, and carboxymethyl cellulose Rhizomucor miehei NRL 3169 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Rhizomucor miehei ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Rhizomucor miehei NRL 3169 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 27000, SDS-PAGE Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
-
 Rhizomucor miehei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 90 activity range Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70 75 purified enzyme, 60 min, completely stable Rhizomucor miehei
3.2.1.8 80
-
 purified enzyme, 60 min, 80% activity remaining Rhizomucor miehei
3.2.1.8 85
-
 purified enzyme, 60 min, 40% activity remaining Rhizomucor miehei
3.2.1.8 90
-
 purified enzyme, half-life is 30 min, 20% activity remaining after 60 min Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5 6.5
-
 Rhizomucor miehei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 4 8.5 activity range Rhizomucor miehei

General Information

EC Number General Information Comment Organism
3.2.1.8 physiological function endoxylanase is the key enzyme for xylan depolymerization, which attacks the main chain of xylan Rhizomucor miehei