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Literature summary extracted from

  • Asai, S.; Mase, K.; Yoshioka, H.
    A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance (2010), Plant J., 62, 911-924.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.12 expressed in Escherichia coli Nicotiana benthamiana

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.25 C396S site-directed mutagenesis, Nicotiana benthamiana
3.5.4.25 E154D site-directed mutagenesis, Nicotiana benthamiana
3.5.4.25 additional information generation of NbRibA-silenced plants via inoculation with Agrobacterium tumefaciens containing inf1, NbMEK2DD or Bax, or GUS as a control. Silencing NbRibA does not affect the activation of SIPK and defense-related genes, except for NbGST, effects of silencing NbRibA on disease resistance, phenotype Nicotiana benthamiana
4.1.99.12 E154D DBPS activity is abolished Nicotiana benthamiana

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.25 Nicotiana benthamiana D6RUS9 guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase; gene NbRibA encoding the bifunctional guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase
-
4.1.99.12 Nicotiana benthamiana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.12 using Ni-NTA chromatography Nicotiana benthamiana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.25 leaf
-
 Nicotiana benthamiana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.12 D-ribulose 5-phosphate
-
 Nicotiana benthamiana formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.25 GTPCH II
-
 Nicotiana benthamiana
3.5.4.25 guanosine triphosphate cyclohydrolase II
-
 Nicotiana benthamiana
3.5.4.25 guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase
-
 Nicotiana benthamiana
3.5.4.25 NbRibA
-
 Nicotiana benthamiana
4.1.99.12 NbRibA bifunctional enzyme: guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase Nicotiana benthamiana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.12 37
-
 assay at Nicotiana benthamiana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.12 8
-
 assay at Nicotiana benthamiana

General Information

EC Number General Information Comment Organism
3.5.4.25 malfunction NbRibA-silenced plants show slightly dwarf phenotypes, levels of endogenous riboflavin and its derivatives FMN and FAD are decreased in NbRibA-silenced Nicotiana benthamiana plants. Silencing of NbRibA compromises not only hypersensitive response cell death, but also the NO and reactive oxygen species production induced by INF1 elicitin and a constitutively active form of NbMEK2, and also induces high susceptibility to oomycete Phytophthora infestans and ascomycete Colletotrichum orbiculare. Compromised radical production and hypersensitive response cell death induced by INF1 in NbRibA-silenced leaves are rescued by adding riboflavin, FMN or FAD Nicotiana benthamiana
3.5.4.25 metabolism the bifunctional enzyme, guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase, participates in the biosynthesis of flavin Nicotiana benthamiana
4.1.99.12 malfunction silencing NbRibA compromises hypersensitive response cell death, NO and ROS production, and induced high susceptibility to oomycete Phytophthora infestans and ascomycete Colletotrichum orbiculare. Compromised radical production and hypersensitive response cell death induced by INF1 in NbRibA-silenced leaves is rescued by adding riboflavin, FMN or FAD Nicotiana benthamiana