EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.83 | overexpression in Catharanthus roseus hairy roots via Agrobacterium rhizogenes 15834 transfection system, co-expression with the 1-deoxy-D-xylulose synthase, DXS, from Arabidopsis thaliana | Catharanthus roseus |
4.1.3.27 | alpha subunit (with a point mutation to confer feedback resistance to tryptophan) expressed in hairy root of Catharanthus roseus | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.83 | additional information | co-overexpression of G10H with the 1-deoxy-D-xylulose synthase, DXS, from Arabidopsis thaliana causes a significant increase in ajmalicine by 16%, lochnericine by 31% and tabersonine by 13%. To increase the flux toward vinblastine and vincristine, a multiple genes within the pathway need to be overexpressed, terpenoid indole alkaloid profile, overview | Catharanthus roseus |
1.14.14.83 | additional information | construction of hairy root lines with inducible overexpression of 1-deoxy-D-xylulose synthase, DXS, or geraniol-10-hydroxylase, G10H | Catharanthus roseus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.83 | Catharanthus roseus | - |
- |
- |
4.1.3.27 | Arabidopsis thaliana | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.83 | hairy root | - |
Catharanthus roseus | - |
4.1.3.27 | hairy root | - |
Arabidopsis thaliana | - |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.83 | G10H | - |
Catharanthus roseus |
1.14.14.83 | geraniol-10-hydroxylase | - |
Catharanthus roseus |
4.1.3.27 | anthranilate synthase | - |
Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.83 | metabolism | the enzyme is part of the terpenoid indole alkaloid pathway | Catharanthus roseus |
1.14.14.83 | additional information | the individual overexpression of the terpenoid genes 1-deoxy-D-xylulose synthase, DXS, and G10H resulted in mixed results in regards to the accumulation of terpenoid indole alkaloid metabolite pools | Catharanthus roseus |