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Literature summary extracted from

  • Goncalves, H.; Riul, A.; Terenzi, H.; Jorge, J.; Guimaraes, L.
    Extracellular tannase from Emericella nidulans showing hypertolerance to temperature and organic solvents (2011), J. Mol. Catal. B, 71, 29-35.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 SDS 138.96% activity at 0.01% (v/v) Aspergillus nidulans
3.1.1.20 Triton X-100 121.63% activity at 0.01% (v/v) Aspergillus nidulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 AgNO3 98.77% residual activity at 1 mM Aspergillus nidulans
3.1.1.20 Al3+ 75.14% residual activity at 1 mM Aspergillus nidulans
3.1.1.20 beta-mercaptoethanol 37.07% residual activity at 20 mM Aspergillus nidulans
3.1.1.20 EDTA 25.89% residual activity at 20 mM Aspergillus nidulans
3.1.1.20 Fe3+ 85.74% residual activity at 1 mM Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 4.78
-
 methyl gallate pH 5.0, 45°C Aspergillus nidulans
3.1.1.20 7.69
-
 propyl gallate pH 5.0, 45°C Aspergillus nidulans
3.1.1.20 14.01
-
 Tannic acid pH 5.0, 45°C Aspergillus nidulans
3.1.1.20 16.94
-
 pyrogallol pH 5.0, 45°C Aspergillus nidulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.20 extracellular
-
 Aspergillus nidulans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ba2+ 117.94% activity at 1 mM Aspergillus nidulans
3.1.1.20 Ca2+ 109.51% activity at 1 mM Aspergillus nidulans
3.1.1.20 Co2+ 133.59% activity at 1 mM Aspergillus nidulans
3.1.1.20 Cu2+ 108.44% activity at 1 mM Aspergillus nidulans
3.1.1.20 Hg2+ 137.73% activity at 1 mM Aspergillus nidulans
3.1.1.20 K+ 115.18% activity at 1 mM Aspergillus nidulans
3.1.1.20 Mg2+ 118.25% activity at 1 mM Aspergillus nidulans
3.1.1.20 Mn2+ 115.8% activity at 1 mM Aspergillus nidulans
3.1.1.20 Na+ 124.69% activity at 1 mM Aspergillus nidulans
3.1.1.20 NH4Cl 110.74% activity at 1 mM Aspergillus nidulans
3.1.1.20 Zn2+ 142% activity at 1 mM Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 45800
-
 2 * 45800 + 2 * 52000, SDS-PAGE Aspergillus nidulans
3.1.1.20 52000
-
 2 * 45800 + 2 * 52000, SDS-PAGE Aspergillus nidulans
3.1.1.20 302000
-
 gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Aspergillus nidulans
-
 gallic acid + D-glucose
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.20 Acetone 130.37% activity at 1% (v/v) Aspergillus nidulans
3.1.1.20 acetonitrile 149.85% activity at 1% (v/v) Aspergillus nidulans
3.1.1.20 Ethanol 148.47% activity at 1% (v/v) Aspergillus nidulans
3.1.1.20 Glycerol 138.5% activity at 1% (v/v) Aspergillus nidulans
3.1.1.20 isopropanol 150% activity at 1% (v/v) Aspergillus nidulans
3.1.1.20 Methanol 139.57% activity at 1% (v/v) Aspergillus nidulans
3.1.1.20 n-Butanol 140.95% activity at 1% (v/v) Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus nidulans
-
 synonym Aspergillus nidulans
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.20 glycoprotein carbohydrate content of 50% Aspergillus nidulans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 DEAE-cellulose column chromatography and Sephacryl S-200 gel filtration Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 0.031
-
 crude extract, pH 5.0, 45°C Aspergillus nidulans
3.1.1.20 1.91
-
 after 61.61fold purification, pH 5.0, 45°C Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 methyl gallate + H2O
-
 Aspergillus nidulans gallate + methanol
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Aspergillus nidulans gallate + propanol
-
 ?
3.1.1.20 pyrogallol + H2O
-
 Aspergillus nidulans ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus nidulans gallic acid + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 heterotetramer 2 * 45800 + 2 * 52000, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 45
-
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 22 90 the enzyme is very stable in the temperature range of 22-50°C for up to 70 h and has a half-life of about 72 h at 90°C Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
-
 Aspergillus nidulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 4 5 the enzyme retained around 80% of control activity when maintained for 60 h at pH 4.0 or 5.0. Increasing pH values (6.0-10.0) result in decreased stability of the enzyme, with a half-life of 20 h at pH 7.0 Aspergillus nidulans

Expression

EC Number Organism Comment Expression
3.1.1.20 Aspergillus nidulans enzyme production is drastically inhibited at higher concentrations of D-glucose (above 0.5% w/v) down
3.1.1.20 Aspergillus nidulans intracellular enzyme levels increase 2fold in the presence of 0.75% (w/v) gallic acid, whereas the addition of low concentrations of D-glucose (0.1% w/v) increase both extra- and intracellular activities up