EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine | - |
Chironomus riparius | |
3.2.1.28 | (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine | - |
Sus scrofa | |
3.2.1.28 | (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine | - |
Chironomus riparius | |
3.2.1.28 | (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine | - |
Sus scrofa | |
3.2.1.28 | 7-deoxycasuarine | - |
Chironomus riparius | |
3.2.1.28 | 7-deoxycasuarine | - |
Sus scrofa | |
3.2.1.28 | 7-homocasuarine | - |
Chironomus riparius | |
3.2.1.28 | 7-homocasuarine | - |
Sus scrofa | |
3.2.1.28 | castanospermine | - |
Chironomus riparius | |
3.2.1.28 | casuarine | - |
Chironomus riparius | |
3.2.1.28 | deoxynojirimycin | inhibition according to a ligand exclusion model | Chironomus riparius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 0.44 | - |
alpha,alpha-trehalose | pH 5.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.48 | - |
alpha,alpha-trehalose | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.52 | - |
alpha,alpha-trehalose | pH 7.5, 30°C | Chironomus riparius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 67000 | - |
gel filtration, PAGE | Chironomus riparius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.28 | Chironomus riparius | - |
- |
- |
3.2.1.28 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.28 | - |
Chironomus riparius |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.28 | kidney | - |
Sus scrofa | - |
3.2.1.28 | larva | - |
Chironomus riparius | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 75 | - |
pH 6.5, 30°C | Sus scrofa |
3.2.1.28 | 75 | - |
pH 6.5, 30°C | Chironomus riparius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Sus scrofa | 2 D-glucose | - |
? | |
3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Chironomus riparius | 2 D-glucose | - |
? | |
3.2.1.28 | additional information | very poor substrates: lactose 0.1%, sucrose 0.08%, cellobiose 0.05%, maltose 0% and raffinose 0.9% of activity against trehalose, respectively | Chironomus riparius | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.28 | monomer | 1 * 67000, SDS-PAGE | Chironomus riparius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 6.5 | - |
- |
Chironomus riparius |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 0.00012 | - |
casuarine | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.00059 | - |
7-deoxycasuarine | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.00139 | - |
deoxynojirimycin | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.00486 | - |
castanospermine | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.0053 | - |
(2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine | pH 6.5, 30°C | Sus scrofa | |
3.2.1.28 | 0.0093 | - |
(2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.0117 | - |
7-deoxycasuarine | pH 6.5, 30°C | Sus scrofa | |
3.2.1.28 | 0.0256 | - |
(2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.033 | - |
7-homocasuarine | pH 6.5, 30°C | Chironomus riparius | |
3.2.1.28 | 0.0554 | - |
(2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine | pH 6.5, 30°C | Sus scrofa | |
3.2.1.28 | 0.107 | - |
7-homocasuarine | pH 6.5, 30°C | Sus scrofa |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 0.00025 | - |
pH 6.5, 30°C | Chironomus riparius | casuarine | |
3.2.1.28 | 0.0012 | - |
pH 6.5, 30°C | Chironomus riparius | 7-deoxycasuarine | |
3.2.1.28 | 0.00283 | - |
pH 6.5, 30°C | Chironomus riparius | deoxynojirimycin | |
3.2.1.28 | 0.00993 | - |
pH 6.5, 30°C | Chironomus riparius | castanospermine | |
3.2.1.28 | 0.0106 | - |
pH 6.5, 30°C | Sus scrofa | (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine | |
3.2.1.28 | 0.019 | - |
pH 6.5, 30°C | Chironomus riparius | (2R,3R,4R)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidine | |
3.2.1.28 | 0.0215 | - |
pH 6.5, 30°C | Sus scrofa | 7-deoxycasuarine | |
3.2.1.28 | 0.0524 | - |
pH 6.5, 30°C | Chironomus riparius | (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine | |
3.2.1.28 | 0.0655 | - |
pH 6.5, 30°C | Chironomus riparius | 7-homocasuarine | |
3.2.1.28 | 0.1108 | - |
pH 6.5, 30°C | Sus scrofa | (2R,3R,4R,5R)-3,4-dihydroxy-2-(hydroxymethyl)-5-methylpyrrolidine | |
3.2.1.28 | 0.214 | - |
pH 6.5, 30°C | Sus scrofa | 7-homocasuarine |