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Literature summary extracted from

  • Zhang, R.; Yang, P.; Huang, H.; Shi, P.; Yuan, T.; Yao, B.
    Two types of phytases (histidine acid phytase and beta-propeller phytase) in Serratia sp. TN49 from the gut of Batocera horsfieldi (Coleoptera) larvae (2011), Curr. Microbiol., 63, 408-415.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.8 Ca2+ activates, dependent on, best at 1 mM, required for thermal stability Serratia sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 gene encoding PhyB49 PCR-amplified from the genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the phytase as N-terminally His6-tagged protein in Escherichia coli strain BL21(DE3) Serratia sp.
3.1.3.8 gene encoding PhyH49 PCR-amplified from the genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the phytase as N-terminally His6-tagged protein in Escherichia coli strain BL21(DE3) Serratia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Ag+
-
 Serratia sp.
3.1.3.8 Cr3+
-
 Serratia sp.
3.1.3.8 EDTA
-
 Serratia sp.
3.1.3.8 Fe3+
-
 Serratia sp.
3.1.3.8 Mg2+
-
 Serratia sp.
3.1.3.8 Mn2+
-
 Serratia sp.
3.1.3.8 Ni2+
-
 Serratia sp.
3.1.3.8 SDS
-
 Serratia sp.
3.1.3.8 Zn2+
-
 Serratia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.14
-
 phytate pH 5.0, 37°C Serratia sp.
3.1.3.8 0.67
-
 phytate pH 7.5, 37°C Serratia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 46000
-
 x * 46000, recombinant His6-tagged phytase PhyH49, SDS-PAGE Serratia sp.
3.1.3.8 66000
-
 x * 66000, recombinant His6-tagged phytase PhyB49, SDS-PAGE Serratia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 phytate + H2O Serratia sp.
-
 ? + phosphate
-
 ?
3.1.3.8 phytate + H2O Serratia sp. TN49, ACCC 03909
-
 ? + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Serratia sp. G4WZU3 acidic histidine acid phosphatase PhyH49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
-
3.1.3.8 Serratia sp. G4WZU4 alkaline beta-propeller phytase PhyB49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
-
3.1.3.8 Serratia sp. TN49, ACCC 03909 G4WZU3 acidic histidine acid phosphatase PhyH49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
-
3.1.3.8 Serratia sp. TN49, ACCC 03909 G4WZU4 alkaline beta-propeller phytase PhyB49; isolated from the gut of Batocera horsfieldi (Coleoptera) larvae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant His6-tagged phytase PhyB49 from Escherichia coli strain BL21(DE3) Serratia sp.
3.1.3.8 recombinant His6-tagged phytase PhyH49 from Escherichia coli strain BL21(DE3) Serratia sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 0.23
-
 recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37°C, in a strain grown under phosphate limitation in the presence of phytate Serratia sp.
3.1.3.8 0.58
-
 recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37°C, in a strain grown under phosphate limitation in the presence of phytate Serratia sp.
3.1.3.8 9.5
-
 purified recombinant alkaline beta-propeller phosphatase PhyH49, pH 7.5, 37°C, substrate phytate Serratia sp.
3.1.3.8 144
-
 purified recombinant acidic histidine phosphatase PhyH49, pH 4.5, 37°C, substrate phytate Serratia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information phytase activity is determined using the ferrous sulfate-molybdenum blue method Serratia sp. ?
-
 ?
3.1.3.8 additional information phytase activity is determined using the ferrous sulfate-molybdenum blue method Serratia sp. TN49, ACCC 03909 ?
-
 ?
3.1.3.8 phytate + H2O
-
 Serratia sp. ? + phosphate
-
 ?
3.1.3.8 phytate + H2O
-
 Serratia sp. TN49, ACCC 03909 ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 46000, recombinant His6-tagged phytase PhyH49, SDS-PAGE Serratia sp.
3.1.3.8 ? x * 66000, recombinant His6-tagged phytase PhyB49, SDS-PAGE Serratia sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 acidic histidine acid phosphatase
-
 Serratia sp.
3.1.3.8 alkaline beta-propeller phytase
-
 Serratia sp.
3.1.3.8 beta-propeller phytase
-
 Serratia sp.
3.1.3.8 histidine acid phytase
-
 Serratia sp.
3.1.3.8 PhyB49
-
 Serratia sp.
3.1.3.8 PhyH49
-
 Serratia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 45
-
 recombinant alkaline beta-propeller phosphatase PhyB49 Serratia sp.
3.1.3.8 60
-
 recombinant acidic histidine phosphatase PhyH49 Serratia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 40
-
 purified recombinant enzyme, stable at Serratia sp.
3.1.3.8 50
-
 the thermal stability of recombinant PhyB49 is affected by the concentration of Ca2+, the recombinant enzyme is stable at 50°C for 30 min at 5 mM Ca2+, but loses activity rapidly under the same conditions in presence of 1 mM Ca2+ Serratia sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 23.46
-
 phytate pH 7.5, 37°C Serratia sp.
3.1.3.8 112.7
-
 phytate pH 5.0, 37°C Serratia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
 recombinant acidic histidine phosphatase PhyH49 Serratia sp.
3.1.3.8 7.5 8 recombinant alkaline beta-propeller phosphatase PhyB49 Serratia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 additional information
-
 the two types of phytases are complementary in phytate degradation over the pH range of pH 2.0-9.0 Serratia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 5 8 purified recombinant enzyme, stable at pH 5.0-8.0, retaining more than 70% of the initial activity at 37°C for 1 h Serratia sp.
3.1.3.8 7 10 purified recombinant enzyme, stable in neutral to alkaline buffers, retaining more than 70% of its activity at pH 7.0-10.0 and 37°C for 1 h Serratia sp.

General Information

EC Number General Information Comment Organism
3.1.3.8 evolution the two phytases, an acidic histidine acid phosphatase, PhyH49, and an alkaline beta-propeller phytase, PhyB49, share low identities with known phytases, 61% at most Serratia sp.
3.1.3.8 additional information two types of phytases in Serratia sp. TN49 Serratia sp.