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Literature summary extracted from

  • Peper, S.; Kara, S.; Long, W.S.; Liese, A.; Niemeyer, B.
    Immobilization and characterization of benzoylformate decarboxylase from Pseudomonas putida on spherical silica carrier (2011), Bioprocess Biosyst. Eng., 34, 671-680.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expressed in Escherichia coli SG13009 cells Pseudomonas putida

General Stability

EC Number General Stability Organism
4.1.1.7 the activity of the spherical silica-immobilized BFD A460I-F464I variant is determined to be 70% related to the activity of the free enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 19.4
-
 benzaldehyde free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida
4.1.1.7 51.9
-
 benzaldehyde immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida
4.1.1.7 515.9
-
 acetaldehyde immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida
4.1.1.7 775
-
 acetaldehyde free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida P20906
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 Q-Sepharose column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 benzaldehyde + acetaldehyde
-
 Pseudomonas putida 2-hydroxypropiophenone
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.7 homotetramer
-
 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.7 45
-
 temperature optimum for immobilized variant A460I/F464I Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate
-
 Pseudomonas putida