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Literature summary extracted from

  • Shin, J.W.; Choi, S.H.; Kim, D.E.; Kim, H.S.; Lee, J.H.; Lee, I.S.; Lee, E.Y.
    Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes (2011), Bioprocess Biosyst. Eng., 34, 113-119.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) at 37°C and at 15°C. The recombinant alginate lyase expressed with pColdI at 15°C exhibits the highest alginate-degrading activity, method evaluation and optimization Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.3 additional information recombinant alginate lyase is efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer is synthesized by the immobilized alginate lyase. The immobilized enzymes can be re-used repeatedly more than 10 times after magnetic separation, overview Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ca2+ activates Streptomyces sp.
4.2.2.3 Co2+ activates Streptomyces sp.
4.2.2.3 Mg2+ activates Streptomyces sp.
4.2.2.3 Mn2+ activates Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 27500
-
 x * 27500, recombinant His-tagged enzyme, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Streptomyces sp.
-
-
-
4.2.2.3 Streptomyces sp. ALG-5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces sp.

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 27500, recombinant His-tagged enzyme, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 50
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 20 80
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 40
-
 activity is stable up to 40°C for 30 min reaction, and then sharply decreases with further increase in temperature Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7 8
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 3.6 11
-
 Streptomyces sp.

General Information

EC Number General Information Comment Organism
4.2.2.3 physiological function the marine alginate lyase from Streptomyces sp. ALG-5 specifically degrades poly-G block of alginate Streptomyces sp.