EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | additional information | reductive activation of the enzyme by ferrdoxin, mechanism, detailed overview | Moorella thermoacetica | |
6.2.1.1 | reduced ferredoxin | required | Moorella thermoacetica |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.169 | expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) from a pet29a(+) vector | Moorella thermoacetica |
6.2.1.1 | expression of C-terminally His-tagged ACS in Escherichia coli strain BL21(DE3) | Moorella thermoacetica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | Cu2+ | - |
Moorella thermoacetica | |
2.3.1.169 | Fe2+ | in cofactor ferredoxin(II), which harbors two [4Fe-4S] clusters | Moorella thermoacetica | |
2.3.1.169 | Zn2+ | - |
Moorella thermoacetica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | Ni2+ | formation of the NiFeC species | Moorella thermoacetica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.169 | acetyl-CoA + corrinoid protein | Moorella thermoacetica | - |
CoA + CO + methylcorrinoid protein | - |
? | |
6.2.1.1 | ATP + acetate + CoA | Moorella thermoacetica | reaction of acetylated ACS with CoA and Fd-II, a step of the catalytic cycle in which the acetylated ACS reacts with CoA to form acetyl-CoA | AMP + diphosphate + acetyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.169 | Moorella thermoacetica | - |
- |
- |
6.2.1.1 | Moorella thermoacetica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.169 | recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under a N2 atmosphere | Moorella thermoacetica |
6.2.1.1 | recombinant C-terminally His-tagged ACS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under anaerobic conditions | Moorella thermoacetica |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.169 | acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] | the methyl and carbonyl groups bind to ACS in a random manner before the strictly ordered binding of the third substrate, CoA, mechanism of acetyl-CoA synthesis by ACS, overview | Moorella thermoacetica |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.3.1.169 | purified recombinant ACS is Ni-reconstituted in the elution buffer with 6 equivalents of NiCl2 for 2 or 3 days at 27°C or at 45°C | Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.169 | acetyl-CoA + corrinoid protein | - |
Moorella thermoacetica | CoA + CO + methylcorrinoid protein | - |
? | |
2.3.1.169 | acetyl-CoA + corrinoid protein | Fd-II can act as a redox mediator by accepting electrons from the acetyl-ACS intermediate and by serving as the initial reducing agent linked to formation of the Ni1+-CO catalytic intermediate | Moorella thermoacetica | CoA + CO + methylcorrinoid protein | - |
? | |
6.2.1.1 | ATP + acetate + CoA | - |
Moorella thermoacetica | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.1 | ATP + acetate + CoA | reaction of acetylated ACS with CoA and Fd-II, a step of the catalytic cycle in which the acetylated ACS reacts with CoA to form acetyl-CoA | Moorella thermoacetica | AMP + diphosphate + acetyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.169 | heterotetramer | CODH and ACS make up the two subunits of a 310 kDa alpha2beta2 heterotetrameric enzymatic complex | Moorella thermoacetica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.1 | Acetyl-CoA synthase | - |
Moorella thermoacetica |
6.2.1.1 | ACS | - |
Moorella thermoacetica |
6.2.1.1 | More | acetyl-CoA synthase is a subunit of the bifunctional CO dehydrogenase/acetyl-CoA synthase, CODH/ACS, complex | Moorella thermoacetica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.169 | 22 | - |
acetyl-CoA exchange assay at room temperature | Moorella thermoacetica |
2.3.1.169 | 45 | - |
methylation reaction assay at | Moorella thermoacetica |
6.2.1.1 | 27 | - |
CO/acetyl-CoA exchange assay at | Moorella thermoacetica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.169 | 6.2 | - |
acetyl-CoA exchange assay at | Moorella thermoacetica |
2.3.1.169 | 7.6 | - |
methylation reaction assay at | Moorella thermoacetica |
6.2.1.1 | 6.2 | - |
CO/acetyl-CoA exchange assay at | Moorella thermoacetica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | acetyl-CoA | - |
Moorella thermoacetica | |
2.3.1.169 | Ferredoxin | Fd-II, which harbors two [4Fe-4S] clusters and is an electron acceptor for CODH, serves as a redox activator of ACS. Catalytic one-electron redox-active species in the CO/acetyl-CoA exchange reaction. Incubation of ACS with Fd-II and CO leads to the formation of the NiFeC species. FdII is purified from Moorella thermoacetica, overview | Moorella thermoacetica |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.169 | physiological function | acetyl-CoA synthase, ACS, a subunit of the bifunctional CO dehydrogenase/acetyl-CoA synthase, CODH/ACS, complex of Moorella thermoacetica requires reductive activation in order to catalyze acetyl-CoA synthesis and related partial reactions, including the CO/acetyl-CoA exchange reaction | Moorella thermoacetica |
6.2.1.1 | metabolism | acetyl-CoA synthase, a subunit of the bifunctional CO dehydrogenase/acetyl-CoA synthase, CODH/ACS, complex of Moorella thermoacetica requires reductive activation in order to catalyze acetyl-CoA synthesis and related partial reactions, including the CO/acetyl-CoA exchange reaction. Ferredoxin(II), which harbors two [4Fe-4S] clusters and is an electron acceptor for CODH, serves as a redox activator of ACS. Ferredoxin interfaces with an internal redox shuttle in acetyl-CoA synthase during reductive activation and catalysis. The midpoint reduction potential for the catalytic one-electron redoxactive species in the CO/acetyl-CoAexchange reaction is -511 mV. Incubation of ACS with Fd-II and CO leads to the formation of the NiFeC species. Mechanism, overview | Moorella thermoacetica |