EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.1 | wild type enzyme is expressed in Escherichia coli SG13009 cells, mutant enzymes E473D and E473Q are expressed in Escherichia coli JM109 cells | Zymomonas mobilis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.1 | hanging drop vapor diffusion method, using 100 mM MES buffer (pH 6.0), 1 mM thiamin diphosphate, 5 mM MgSO4, and 20-24% (w/v) polyethylene glycol 1500 | Zymomonas mobilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.1 | E473D | the mutant exhibits a residual activity of 0.6% compared to the wild type enzyme, wild type PDC and the Glu473Asp variant bind the substrate analogue acetylphosphinate with the same affinity | Zymomonas mobilis |
4.1.1.1 | E473Q | the mutant exhibits a residual activity of 0.1% compared to the wild type enzyme, Glu473Gln fails to bind the substrate analogue acetylphosphinate | Zymomonas mobilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.1 | 0.15 | - |
pyruvate | mutant enzyme E473D, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis | |
4.1.1.1 | 0.31 | - |
pyruvate | wild type enzyme, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis | |
4.1.1.1 | 0.4 | - |
pyruvate | mutant enzyme E473Q, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.1 | Zymomonas mobilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.1 | Fractogel EMD TMAE column chromatography and Superdex 200 gel filtration | Zymomonas mobilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.1 | acetylphosphinate | - |
Zymomonas mobilis | ? | - |
? | |
4.1.1.1 | pyruvate | - |
Zymomonas mobilis | acetaldehyde + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.1 | PDC | - |
Zymomonas mobilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.1 | 0.1 | - |
pyruvate | mutant enzyme E473D, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis | |
4.1.1.1 | 0.15 | - |
pyruvate | mutant enzyme E473Q, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis | |
4.1.1.1 | 150 | - |
pyruvate | wild type enzyme, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.1 | thiamine diphosphate | - |
Zymomonas mobilis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.1 | 0.38 | - |
pyruvate | mutant enzyme E473Q, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis | |
4.1.1.1 | 0.67 | - |
pyruvate | mutant enzyme E473D, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis | |
4.1.1.1 | 484 | - |
pyruvate | wild type enzyme, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate | Zymomonas mobilis |