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Literature summary extracted from

  • Shangguan, J.J.; Liu, Y.Q.; Wang, F.J.; Zhao, J.; Fan, L.Q.; Li, S.X.; Xu, J.H.
    Expression and characterization of a novel lipase from Aspergillus fumigatus with high specific activity (2011), Appl. Biochem. Biotechnol., 165, 949-962.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 PEG 20000 124% activity at 0.1% (q/v) Aspergillus fumigatus
3.1.1.3 polyvinyl alcohol 118% activity at 0.1% (w/v) Aspergillus fumigatus
3.1.1.3 Triton X-100 128% activity at 0.1% (v/v) Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Escherichia coli BL21(DE3) cells Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 D260A the mutant shows reduced activity compared to the wild type enzyme Aspergillus fumigatus
3.1.1.3 D264A the mutant shows reduced activity compared to the wild type enzyme Aspergillus fumigatus
3.1.1.3 H283A the mutant shows reduced activity compared to the wild type enzyme Aspergillus fumigatus
3.1.1.3 H290A the mutant shows reduced activity compared to the wild type enzyme Aspergillus fumigatus
3.1.1.3 S165A the mutant shows reduced activity compared to the wild type enzyme Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cu2+ 47.5% residual activity at 2 mM Aspergillus fumigatus
3.1.1.3 Fe2+ 57.2% residual activity at 2 mM Aspergillus fumigatus
3.1.1.3 Li+ 76.4% residual activity at 1 mM Aspergillus fumigatus
3.1.1.3 Mg2+ 85% residual activity at 2 mM Aspergillus fumigatus
3.1.1.3 additional information not inhibited by Ca2+, Mn2+, Ni2+, Ba2+, and EDTA Aspergillus fumigatus
3.1.1.3 SDS 0.201% residual activity at 0.1% (w/v) Aspergillus fumigatus
3.1.1.3 Zn2+ 32.2% residual activity at 2 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 14
-
 4-nitrophenyl acetate at 30°C, pH 7.5 Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information metal ions cannot activate the lipase Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 38000
-
 x * 38000, SDS-PAGE Aspergillus fumigatus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 1-butanol 0.917% residual activity at 50% (v/v), 4.28% residual activity at 10% (v/v) Aspergillus fumigatus
3.1.1.3 Acetone 0.917% residual activity at 50% (v/v), 113% activity at 10% (v/v) Aspergillus fumigatus
3.1.1.3 DMSO 57.2% residual activity at 50% (v/v), 99.1% activity at 10% (v/v) Aspergillus fumigatus
3.1.1.3 Ethanol 2.14% residual activity at 50% (v/v), 100% activity at 10% (v/v) Aspergillus fumigatus
3.1.1.3 isopropanol no activity at 50% (v/v), 83.8% activity at 10% (v/v) Aspergillus fumigatus
3.1.1.3 Methanol 0.613% residual activity at 50% (v/v), 108% activity at 10% (v/v) Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Aspergillus fumigatus
-
-
-
3.1.1.3 Aspergillus fumigatus CGMCC 2873
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 Ni-NTA agarose column chromatography Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 mycelium
-
 Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 118
-
 supernatant of cell lysate, using 4-nitrophenyl acetate as substrate, at 30°C, pH 7.5 Aspergillus fumigatus
3.1.1.3 1000
-
 after 8.47fold purification, using 4-nitrophenyl acetate as substrate, at 30°C, pH 7.5 Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O 100% activity Aspergillus fumigatus 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O 100% activity Aspergillus fumigatus CGMCC 2873 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O about 65% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O about 65% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus CGMCC 2873 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O about 10% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Aspergillus fumigatus 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O worst substrate, less than 5% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl propionate + H2O about 60% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus 4-nitrophenol + propionate
-
 ?
3.1.1.3 4-nitrophenyl propionate + H2O about 60% activity compared to 4-nitrophenyl acetate Aspergillus fumigatus CGMCC 2873 4-nitrophenol + propionate
-
 ?
3.1.1.3 additional information AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate Aspergillus fumigatus ?
-
 ?
3.1.1.3 additional information almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate Aspergillus fumigatus ?
-
 ?
3.1.1.3 additional information AFL1-1 has potential enantioselectivity for the ability to catalyze methyl 3-(4-methoxyphenyl) glycidate and methyl 3-phenylglycidate Aspergillus fumigatus CGMCC 2873 ?
-
 ?
3.1.1.3 additional information almost no activity on long-chained acyl esters such as 4-nitrophenyl palmitate Aspergillus fumigatus CGMCC 2873 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 38000, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 AFL1-1 recombinant enzyme Aspergillus fumigatus
3.1.1.3 lipase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 65
-
 above the optimal temperature, the activity decreases sharply with almost 80% loss of its original activity at 75°C Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30 50 the enzyme is stable (60% activity) at 30-50 °C for 1 h Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.5
-
-
 Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 3 10 at pH 7.0, the activity remains at nearly 100% of the maximal activity, and drops dramatically at pH below 9.0 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6 8.5 the enzyme is stable in the pH range 6.0-8.5 for 16 h (at 4 °C) Aspergillus fumigatus