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Literature summary extracted from
- A new steroid-transforming strain of Mycobacterium neoaurum and cloning of 3-ketosteroid 9alpha-hydroxylase in NwIB-01 (2010), Appl. Biochem. Biotechnol., 162, 1446-1456.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.99.24 | additional information | strain NwIB-01 can be applied as excellent phytosterols-transformation strains in potential industrial applications | Mycolicibacterium neoaurum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.24 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree | Mycolicibacterium neoaurum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.30 | 45370 | - |
x * 45370, calculated from amino acid sequence | Mycolicibacterium neoaurum |
| 1.14.99.24 | 45370 | - |
x * 45370, sequence calculation | Mycolicibacterium neoaurum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.24 | additional information | Mycolicibacterium neoaurum | KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione | ? | - |
? |  |
| 1.14.99.24 | additional information | Mycolicibacterium neoaurum NwIB-01 | KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.30 | Mycolicibacterium neoaurum | C9E9N6 | - |
- |
| 1.14.15.30 | Mycolicibacterium neoaurum NwIB-01 | C9E9N6 | - |
- |
| 1.14.99.24 | Mycolicibacterium neoaurum | C9E9N6 | - |
- |
| 1.14.99.24 | Mycolicibacterium neoaurum NwIB-01 | C9E9N6 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.30 | androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycolicibacterium neoaurum | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
| 1.14.15.30 | androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Mycolicibacterium neoaurum NwIB-01 | 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
| 1.14.99.24 | additional information | KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione | Mycolicibacterium neoaurum | ? | - |
? | |
| 1.14.99.24 | additional information | KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione | Mycolicibacterium neoaurum NwIB-01 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.30 | ? | x * 45370, calculated from amino acid sequence | Mycolicibacterium neoaurum |
| 1.14.99.24 | ? | x * 45370, sequence calculation | Mycolicibacterium neoaurum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.30 | 3-ketosteroid 9alpha-hydroxylase | - |
Mycolicibacterium neoaurum |
| 1.14.15.30 | KSH | - |
Mycolicibacterium neoaurum |
| 1.14.99.24 | 3-ketosteroid 9alpha-hydroxylase | - |
Mycolicibacterium neoaurum |
| 1.14.99.24 | KSH | - |
Mycolicibacterium neoaurum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.15.30 | Mycolicibacterium neoaurum | calculated from amino acid sequence | - |
5.5 |
| 1.14.99.24 | Mycolicibacterium neoaurum | sequence calculation | - |
5.46 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.15.30 | metabolism | the enzyme is essential for the steroid metabolism | Mycolicibacterium neoaurum |
| 1.14.99.24 | metabolism | key enzyme in steroid degradation. The two key enzymes, 3-ketosteroid DELTA1-dehydrogenase, KsdD, and 3-ketosteroid 9alpha-hydroxylase, KSH, are essential for the production and accumulation of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione from soy bean, Glycine max | Mycolicibacterium neoaurum |
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Release 2023.1 (January 2023)
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