BRENDA - Enzyme Database show

Oxidosqualene Cyclase Residues that Promote Formation of Cycloartenol, Lanosterol, and Parkeol We are grateful to Bridget M. Joubert for advice regarding mutagenesis. We thank Elizabeth A. Hart for an authentic parkeol standard, and for chromatographic and spectroscopic information. This research was funded by the National Institutes of Health (grant no. AI 41598) and the Robert A. Welch Foundation (grant no. C-1323). M.M.M. was an American Society of Pharmacognosy Undergraduate Fellow. M.J.R.S. was a Robert A. Welch Fellow and was supported by an NIH Biotechnology Training Grant (grant no. T32 GM08362).

Khenkin, A.; Neumann, R.; Angew. Chem. 39, 4090-4092 (2000)

Data extracted from this reference:

Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.47
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.47
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y produces 11% parkeol, 79% lanosterol and 10% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
5.4.99.47
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V4545L produces 60% parkeol, 20% lanosterol and 20% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
5.4.99.47
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V454I produces 64% parkeol, 13% lanosterol and 23% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.47
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y produces 11% parkeol, 79% lanosterol and 10% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
5.4.99.47
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V4545L produces 60% parkeol, 20% lanosterol and 20% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?
5.4.99.47
(S)-2,3-oxidosqualene
-
713760
Saccharomyces cerevisiae
parkeol + lanosterol + lanost-24-ene-3beta,9alpha-diol
lanosterol-synthase mutant T384Y/V454I produces 64% parkeol, 13% lanosterol and 23% lanost-24-ene-3beta,9alpha-diol from (S)-2,3-oxidosqualene
-
-
?